Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.26.4 (RNase H)
 2,751 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Antibodies directed against reverse transcriptases (RT) of the mammalian retroviruses SSV and RD114 recognize specifically on Western Blots yeast cytoplasmic soluble proteins of 31 and 45 kDa respectively and inhibit RT activity in this fraction. Anti RLV RTIgG recognizes a protein of 40-43 kDa in the particulate (VLP) fraction and inhibits RT activity in it. No inhibition of RT activity was seen with normal serum anti AMV RTIgG or antisera against yeast DNA polymerase I and DNA polymerase III. These yeast RTs display RNase H activity and are not inhibited by aphidicolin. They prefer Mn+2 as cofactor over Mg+2, display an optimum temperature of 25-30 degrees C and are expressed in a diploid as well as 2 haploid strains and are thus distinct from yeast Ty encoded RTs.
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PMID:Some yeast proteins are recognized by antibodies to reverse transcriptases from several mammalian retroviruses and display reverse transcriptase activity. 171 50

Virally regulated HIV-1 particles were expressed from DNA plasmids encoding Gag, protease, reverse transcriptase, Vpu, Tat, Rev, and Env. The sequences for integrase, Vpr, Vif, Nef, and the long terminal repeats (LTRs) were deleted. Mutations were engineered into the VLP genome to produce particles deficient in activities associated with viral reverse transcriptase, RNase H, and RNA packaging. Each plasmid efficiently secreted particles from primate cells in vitro and particles were purified from the supernatants and used as immunogens. Mice (BALB/c) were vaccinated intranasally (day 1 and weeks 3 and 6) with purified VLPs and the elicited immunity was compared to particles without Env (Gag(p55)), to soluble monomeric Env(gp120), or to soluble trimerized Env(gp140). Only mice vaccinated with VLPs had robust anti-Env cellular immunity. In contrast, all mice had high titer anti-Env serum antibody (IgG). However, VLP-vaccinated mice had antisera that detected a broader number of linear Env peptides, had anti-Env mucosal IgA and IgG, as well as higher titers of serum neutralizing antibodies. VLPs elicited high titer antibodies that recognized linear regions in V4-C5 and the ectodomain of gp41, but did not recognize V3. These lentiviral VLPs are effective mucosal immunogens that elicit broader immunity against Env determinants in both the systemic and mucosal immune compartments than soluble forms of Env.
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PMID:Membrane embedded HIV-1 envelope on the surface of a virus-like particle elicits broader immune responses than soluble envelopes. 1701 Oct 11