Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.4 (
RNase H
)
2,751
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
TEP1
is a protein component of two ribonucleoprotein complexes: vaults and telomerase. The vault-associated small RNA, termed vault RNA (VR), is dependent upon
TEP1
for its stable association with vaults, while the association of telomerase RNA with the telomerase complex is independent of
TEP1
. Both of these small RNAs have been shown to interact with amino acids 1-871 of
TEP1
in an indirect yeast three-hybrid assay. To understand the determinants of
TEP1
-RNA binding, we generated a series of
TEP1
deletions and show by yeast three-hybrid assay that the entire Tetrahymena p80 homology region of
TEP1
is required for its interaction with both telomerase and VRs. This region is also sufficient to target the protein to the vault particle. Electrophoretic mobility shift assays using the recombinant
TEP1
RNA-binding domain (TEP1-RBD) demonstrate that it binds RNA directly, and that telomerase and VRs compete for binding. VR binds weakly to
TEP1
-RBD in vitro, but mutation of VR sequences predicted to disrupt helices near its central loop enhances binding. Antisense oligonucleotide-directed
RNase H
digestion of endogenous VR indicates that this region is largely single stranded, suggesting that
TEP1
may require access to the VR central loop for efficient binding.
...
PMID:The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle. 1570 61
