Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.26.4 (RNase H)
 2,751 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The expression of the mRNA for mouse testicular lactate dehydrogenase (LDH-X) was examined by RNA:cDNA hybridization in situ in the testis and by Northern analyses of meiotic and postmeiotic spermatogenic cell populations. Silver grains accumulated in cells inside the second layer from the periphery of the seminiferous tubule, confirming previous findings that LDH-X mRNA first appears in the spermatocyte and continues to accumulate until the late spermatid stage. Northern analyses showed that meiotic and postmeiotic cells contained 1.2 and 1.3 kb classes of hybridizing mRNA, respectively. RNase H digestion of oligo (dT)-hybridized RNA and poly(U)-Sepharose column chromatography with differential elution by formamide revealed that the difference in size of the two classes of mRNAs was due to the poly(A) tail length of the LDH-X mRNA. When the distribution of the LDH-X mRNA was examined across polysome gradients, both mRNAs were partially associated with polysomes. These results suggest that the changes in the polyadenylation of LDH-X mRNA were associated with the meiotic division during spermatogenesis in the mouse. They raise the possibility that the stable accumulation of the LDH-X mRNAs in the postmeiotic cells is enhanced by poly(A) tails of increased length.
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PMID:Changes in polyadenylation of lactate dehydrogenase-X mRNA during spermatogenesis in mice. 290 41

A well-designed DNA tetrahedron based biosensor (DTB) has been employed for imaging and detection of argonaute2 (Ago2), a key RNA interference (RNAi) protein. This DTB mainly contains two segments: DNA tetrahedron as a frame and a photoinduced electron-transfer (PET) pair as a fluorescence transducer. The DNA tetrahedral nanostructure is assembled with four nucleic acid strands. On one edge, one DNA strand forms a hairpin structure (RNA sequence) which is implanted in the two complementary double-strand DNAs, and the PET pairs, DNA/silver nanocluster (AgNC) and G-quadruplex/hemin complex, are labeled at the two termini of another DNA strand, respectively. The DNA tetrahedron structure forms a switchable scaffold that can present the functional DNA motifs in two different modes, according to the presence/absence of the target protein. The cleavage reaction by Ago2/miR-21 complex opens the hairpin structure, leading the PET pairs to be separated to each other in the spatial state. Thus, the DNA/AgNC fluorescence can be measured. By using this DTB, we obtained the 4.54 nM Ago2 detection limit and successful assay of Ago2/miR-21 complex in living cells. Also, the DTB was successfully employed for the further assay of RNase H, which plays an important role in the human immunodeficiency virus type-1 (HIV-1) reverse transcriptase pathway, with a limit of detection (LOD) of 3.41 U mL-1. Our DTB can be used as a device for the imaging protein concentration in single cells and has a potential value for disease diagnosis and treatment.
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PMID:DNA Tetrahedron Based Biosensor for Argonaute2 Assay in Single Cells and Human Immunodeficiency Virus Type-1 Related Ribonuclease H Detection in Vitro. 3105 Aug 88