Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.4 (
RNase H
)
2,751
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Antisense DNAs complementary against various sequences of the
alpha-sarcin
domain (C2646-G2674) of 23S rRNA from Escherichia coli were hybridized to naked 23S rRNA as well as to 70S ribosomes. Saturation levels of up to 0.4 per 70S ribosome were found, the identical fraction was susceptible to the attack of the RNase
alpha-sarcin
. The hybridization was specific as demonstrated with
RNase H
digestion, sequencing the resulting fragments and blockage of the action of
alpha-sarcin
. The RNase
alpha-sarcin
seems to approach its cleavage site from the 3' half of the loop of the
alpha-sarcin
domain. Hybridization is efficiently achieved at 37 degrees C and can extend at least into the 3' strand of the stem of the
alpha-sarcin
domain. However, the inhibition of
alpha-sarcin
activity is observed at 30 degrees C but not at 37 degrees C. For a significant inhibition of poly(Phe) synthesis the temperature had to be lowered to 25 degrees C. The results imply that the
alpha-sarcin
domain changes its conformation during protein synthesis and that the conformational changes may include a melting of the stem of the
alpha-sarcin
domain.
...
PMID:Effects of antisense DNA against the alpha-sarcin stem-loop structure of the ribosomal 23S rRNA. 891 3
