Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.4 (
RNase H
)
2,751
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In order to design ribozymes for the efficient cleavage of a human
acetylcholinesterase
(
AChE
) in vitro transcript, a completely randomized decadeoxyribonucleotide (dN10) was used in conjunction with
RNase H
to identify suitable sites for annealing. Based on the observed cleavage pattern, ribozymes were designed to cleave the transcript at these positions. Five ribozymes so designed proved to be efficient in the transcript cleavage (k(react)/Km ranged from 0.9 x 10(4) to 68.2 x 10(4) M(-1) min(-10)). The best was 150-fold more active than the best designed on the basis of the MFold program. Thus, the
RNase H
mapping demonstrated a high predictive power for favorable ribozyme cleavage sites. The digestion pattern with
RNase H
differed dramatically from that observed with the single-strand-specific mung bean nuclease.
...
PMID:Probing accessible sites for ribozymes on human acetylcholinesterase RNA. 908 49
