Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.4 (
RNase H
)
2,751
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The SCO2299 gene from Streptomyces coelicolor encodes a single peptide consisting of 497 amino acid residues. Its N-terminal region shows high amino acid sequence similarity to RNase HI, whereas its C-terminal region bears similarity to the CobC protein, which is involved in the synthesis of cobalamin. The SCO2299 gene suppressed a temperature-sensitive growth defect of an Escherichia coli
RNase H
-deficient strain, and the recombinant SCO2299 protein cleaved an RNA strand of RNA.DNA hybrid in vitro. The N-terminal domain of the SCO2299 protein, when overproduced independently, exhibited
RNase H
activity at a similar level to the full length protein. On the other hand, the C-terminal domain showed no CobC-like activity but an
acid phosphatase
activity. The full length protein also exhibited
acid phosphatase
activity at almost the same level as the C-terminal domain alone. These results indicate that
RNase H
and
acid phosphatase
activities of the full length SCO2299 protein depend on its N-terminal and C-terminal domains, respectively. The physiological functions of the SCO2299 gene and the relation between
RNase H
and
acid phosphatase
remain to be determined. However, the bifunctional enzyme examined here is a novel style in the Type 1
RNase H
family. Additionally, S. coelicolor is the first example of an organism whose genome contains three active
RNase H
genes.
...
PMID:The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain. 1594 15
