Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.4 (
RNase H
)
2,751
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The avian RNA tumour virus structural protein
p12
was purified from avian myeloblastosis virus (AMV) by nucleic acid affinity chromatography to apparent homogeneity as judged from SDS--polyacrylamide gel electrophoresis. A filter binding assay was used for the identification of
p12
. High concentrations of
p12
precipitated nucleic acids out of solution in the absence of MgCl2. Binding of
p12
to single-stranded nucleic acids protected them from digestion with nucleases and resulted in a hyperchromic effect. These phenomena were reversible in the presence of salt. The affinity of
p12
to nucleic acids was determined by competing for the binding of
p12
to denatured radioactive DNA by various other nuclei acids. It was found that
p12
bound preferentially to single-stranded nucleic acids and showed a higher affinity to poly(rI) than to poly(rC) and poly(rA). Purified RNA-dependent DNA polymerase activity from AMV was stimulated up to sixfold by
p12
, depending on the template. Solubilization of RNA in RNA--DNA hybrids by
RNase H
was inhibited in the presence of
p12
.
...
PMID:Properties of the avian viral protein p12. 616 96
