Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.3 (
RNase III
)
1,015
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Anoxygenic photosynthesis is an important pathway for
Rhodobacter sphaeroides
to produce ATP under oxygen-limiting conditions. The expression of its photosynthesis genes is tightly regulated at transcriptional and post-transcriptional levels in response to light and oxygen signals, to avoid photooxidative stress by the simultaneous presence of pigments, light and oxygen. The
puf
operon encodes pigment-binding proteins of the light-harvesting complex I (genes
pufB
and
pufA
), of the reaction center (genes
pufL
and
pufM
), a scaffold protein (gene
pufX
) and includes the gene for sRNA PcrX. Segmental differences in the stability of the
pufBALMX-pcrX
mRNA contribute to the stoichiometry of LHI to RC complexes. With asPcrL we identified the third sRNA and the first antisense RNA that is involved in balancing photosynthesis gene expression in
R. sphaeroides
. asPcrL influences the stability of the
pufBALMX-pcrX
mRNA but not of the
pufBA
mRNA and consequently the stoichiometry of photosynthetic complexes. By base pairing to the
pufL
region asPcrL promotes
RNase III
-dependent degradation of the
pufBALMX-prcX
mRNA. Since asPcrL is activated by the same protein regulators as the
puf
operon including PcrX it is part of an incoherent feed-forward loop that fine-tunes photosynthesis gene expression. [Figure: see text].
...
PMID:Antisense RNA asPcrL regulates expression of photosynthesis genes in
Rhodobacter sphaeroides
by promoting RNase III-dependent turn-over of
puf
mRNA. 3325 5
