Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.3 (
RNase III
)
1,015
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The rRNA genes of
Borrelia
(
Borreliella
)
burgdorferi
are unusually organized; the spirochete has a single 16S rRNA gene that is more than 3 kb from a tandem pair of 23S-5S rRNA operons. We generated an
rnc
null mutant in
B. burgdorferi
that exhibits a pleiotropic phenotype, including decreased growth rate and increased cell length. Here, we demonstrate that endoribonuclease III (
RNase III
) is, as expected, involved in processing the 23S rRNA in
B. burgdorferi
The 5' and 3' ends of the three rRNAs were determined in the wild type and
rnc
Bb
mutants; the results suggest that
RNase III
in
B. burgdorferi
is required for the full maturation of the 23S rRNA but not for the 5S rRNA nor, curiously, for the 16S rRNA.
IMPORTANCE
Lyme disease, the most common tick-borne
zoonosis
in the Northern Hemisphere, is caused by the bacterium
Borrelia
(
Borreliella
)
burgdorferi
, a member of the deeply branching spirochete phylum.
B. burgdorferi
carries a limited suite of ribonucleases, enzymes that cleave RNA during processing and degradation. Several ribonucleases, including
RNase III
, are involved in the production of ribosomes, which catalyze translation and are a major target of antibiotics. This is the first study to dissect the role of an RNase in any spirochete. We demonstrate that an
RNase III
mutant is viable but has altered processing of rRNA.
...
PMID:RNase III Processing of rRNA in the Lyme Disease Spirochete Borrelia burgdorferi. 2963 96
