Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.25.1 (
deoxyribonuclease
)
1,471
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Cofilin promotes the depolymerization of actin filaments, which is required for a variety of cellular responses such as the formation of lamellipodia and chemotaxis. Phosphorylation of
cofilin
on serine residue 3 is known to block these activities. We now report that neutrophils contain a protein kinase that selectively catalyzes the phosphorylation of
cofilin
on serine 3 (>/=70%) and a nonspecific kinase that recognizes multiple sites in this protein. The selective serine 3
cofilin
kinase binds to a deoxyribonuclease I affinity column, whereas the nonspecific
cofilin
kinase does not. Deoxyribonuclease I forms a very tight complex with actin, and
deoxyribonuclease
affinity columns have been utilized to identify a variety of proteins that interact with the cytoskeleton. The serine 3
cofilin
kinase did not react with antibodies to LIM kinase 1 or 2, which can catalyze the phosphorylation of
cofilin
in other cell types. The activity of the serine 3
cofilin
kinase was insensitive to a variety of selective antagonists of protein kinases but was blocked by staurosporine. This pattern of inhibition is similar to that observed for the kinase that is active with
cofilin
in intact neutrophils. Thus, neutrophils contain a protein kinase distinct from LIM kinase-1/2 that selectively recognizes serine 3 in
cofilin
.
...
PMID:A protein kinase from neutrophils that specifically recognizes Ser-3 in cofilin. 1064 54
