Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.25.1 (
deoxyribonuclease
)
1,471
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A thermophilic bacterial strain, Streptomyces thermonitrificans, produced high levels of extracellular
deoxyribonuclease
(
DNase
) when grown on
NBG
medium (containing 1% peptone, 0.3% beef extract, 1% glucose and 0.5% NaCl). Maximum
DNase
activity (140 U x ml(-1)) was obtained, in 24 h, when the culture was grown on modified
NBG
medium (containing 1.3% beef extract, 1% glucose, 0.5% NaCl and 50 microM Mn2+ at 45 degrees C. The crude enzyme showed higher activity on native DNA than on sonicated and heat denatured DNA. Moreover, addition of Mn2+ in the assay mixture resulted in a significant stimulation (10-15 fold) of the enzyme activity.
...
PMID:Extracellular deoxyribonuclease production by a thermophile, Streptomyces thermonitrificans. 1045 6
