Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.25.1 (deoxyribonuclease)
 1,471 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A considerable amount of Mn2+-stimulated DNAase (deoxyribonuclease) activity is released by Bacillus subtilis 168 during sporulation in a glucose-deficient medium; much smaller amounts are released during starvation for phosphate or nitrogen. Protein synthesis is required. Two forms of evidence are presented that production of the DNAase is associated with events late in stage II of sporulation. 19 Thymidine starvation, which inhibits the biochemical events associated with sporulation, also inhibits release of the DNAase. 2. Several asporogenous mutants blocked at stage II or earlier and unable to produce alkaline phosphatase (a stage-II event) do not produce the enzyme. Mutants blocked towards the end of stage II or later produce both enzymes. During sporulation of the wild-type strain, the DNAase appears about 1 h after alkaline phosphatase. The results suggest that production of the DNAase is controlled by a still-undiscovered stage-II genetic locus.
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PMID:Extracellular manganese-stimulated deoxyribonuclease as a marker event in sporulation of Bacillus subtilis. 41 78

The association between selected neuropathological lesions and effects on mitochondrial and nuclear DNA synthesis was explored in cats exposed in vivo to methylmercuric chloride. Two groups of eight adult female cats ingested 0 or 176 micrograms Hg/kg body wt/day as methylmercuric chloride added daily to their diet. Treated animals and concurrent controls were sacrificed following the onset of clinical signs of toxicity, with the mean termination time being about 12 (range 7-15) weeks. Terminal Hg levels for the control and treated groups respectively were 0.16 +/- 0.02 and 12 +/- 1 ppm in the cerebrum and 0.16 +/- 0.01 and 14 +/- 1 ppm in blood. Hydroxyurea-resistant [3H]thymidine incorporation into DNA in cultured explants of cerebrum and cerebellum, as measured by scintillation counting of extracted DNA, was elevated for treated animals. Autoradiographic analysis indicated that the excess DNA synthesis was cytoplasmic, and deoxyribonuclease resistant, suggesting a mitochondrial DNA origin. The excess DNA synthesis was pronounced in cell types prone to neurodegeneration, specifically the Purkinje cells and the granular cell layer in the cerebellum and the large neurons in the cerebrum. Mitochondrial DNA from neural tissues of an additional five pairs of cats treated for 8 weeks was isolated from cesium chloride/ethidium bromide density gradients. Thymidine incorporation into mitochondrial DNA was greater in methylmercury-treated than control animals. These observations indicate that methylmercury affects mitochondrial DNA synthesis in vivo with a tissue specificity parallel to that of neuropathological lesions.
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PMID:Methylmercury-induced mitochondrial DNA synthesis in neural tissue of cats. 398 97

(3H) Thymidine is incorporated into some cerebellar Purkinje cells of 6- to 30-day-old rats. The frequency of labelled neuronal nuclei was higher in the 12- to 30-day old rats than in the 6- to 10-day-old animals. The grain distribution pattern in autoradiographs was mostly nucleolar amounting to three to ten grains. Some other local labels were revealed, too. Only six Purkinje cells among 42,000 studied in 21 rats possessed heavy label (25 to 50 grains) distributed throughout the nucleus. Control estimations with deoxyribonuclease, hot perchloric acid and covering the autoradiographs again established that the Purkinje cells synthesize DNA perhaps for the purpose of DNA surplus accumulation and/or DNA repair in the neurons.
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PMID:DNA synthesis in the Purkinje neurons. 647 76