Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.25.1 (
deoxyribonuclease
)
1,471
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The small heat-shock proteins appear to have a regulatory role in actin dynamics. Since cytoskeletal disruption is integral to ischemic renal injury, we evaluated expression and intracellular distribution of heat-shock protein 25 (HSP-25) in rat renal cortex after 45 min of renal ischemia.
HSP
-25 was constitutively expressed and induced by ischemia with peak levels reached by 6 h reflow. Ischemia caused a shift of
HSP
-25 from the detergent-soluble into the insoluble cytoskeletal fraction. By 2 h reflow, the majority of
HSP
-25 had redistributed into the soluble fraction.
HSP
-25 was predominantly localized in a subapical distribution in control proximal tubules, a pattern intermediate between
deoxyribonuclease
(
DNase
)-reactive and filamentous actin. After ischemia,
HSP
-25 dispersed through the cytoplasm with small punctate accumulations similar to
DNase
-reactive actin. During later reflow, all three proteins were found in coarse intracytoplasmic accumulations; however,
HSP
-25 and
DNase
-reactive actin were in separate accumulations.
HSP
-25 and microfilamentous actin staining returned to the subapical domain. Thus the temporal and spatial patterns of
HSP
-25 induction and distribution suggest specific interactions between
HSP
-25 and actin during the early postischemic reorganization of the cytoskeleton.
HSP
-25 may have additional roles distinct from actin dynamics later in the course of postischemic recovery.
...
PMID:Heat-shock protein 25 induction and redistribution during actin reorganization after renal ischemia. 945 42
