Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.25.1 (
deoxyribonuclease
)
1,471
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The Epstein-Barr virus (EBV) alkaline deoxyribonuclease (
DNase
) was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV). Infection of the insect cell line Spodoptera frugiperda (SF9) with the recombinant virus led to the expression of an enzymatically active alkaline DNase. The recombinant EBV alkaline DNase was highly soluble, and the recombinant baculovirus produced approximately 10-20 mg of EBV
DNase
per 1 X 10(9) cells. The recombinant enzyme activity was neutralized by specific antisera to the EBV
DNase
and was recognized by these sera in Western blot analysis and immunofluorescence tests. The recombinant EBV
DNase
was neutralized by these sera from patients with nasopharyngeal carcinoma and chronic
infectious mononucleosis
. Western blot analysis using these patients' sera showed that IgG and IgA antibodies to the EBV
DNase
could be readily detected.
...
PMID:High-level expression of the Epstein-Barr virus alkaline deoxyribonuclease using a recombinant baculovirus: application to the diagnosis of nasopharyngeal carcinoma. 184 61
