Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.22.1 (DNase II)
 429 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Differentiation of cartilage from precartilage mesenchyme in the chick embryo is accompanied by the loss of two abundant nonhistone proteins (Mr 35 500 and 125 000) termed PCP 35.5 and PCP 125. Here we examine the distribution of these and other developmentally regulated nonhistones in nuclease-sensitive regions of precartilage and cartilage chromatin. In particular, we show that PCP 35.5 is a tight DNA-binding protein that is localized near deoxyribonuclease I (DNase I) sensitive regions of precartilage chromatin. Localization of nonhistones was demonstrated by excising domains of precartilage chromatin with DNase II which are simultaneously highly enriched in PCP 35.5, in PCP 125, and DNase I sensitive DNA sequences. These domains comprise at least 25% of the cell's DNase I sensitive sequences, as well as small DNase I resistant regions with which the two nonhistones are associated. These findings suggest that PCP 35.5 (and possibly PCP 125) may play a developmentally regulated role nearby DNase I sensitive domains of the cartilage progenitor cell chromatin.
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PMID:Developmentally regulated nonhistone proteins: evidence for deoxyribonucleic acid binding role and localization near deoxyribonuclease I sensitive domains of precartilage cell chromatin. 628 99

The adenovirus-specific DNA-binding protein (DBP) has been shown to inhibit the hydrolysis of single-stranded DNA by a DNase isolated from KB cells, (Nass, K., and Frenkel, G.D. (1980). J. Virol. 35, 314-319). The specificity of the inhibition has now been investigated. The DBP inhibits the hydrolysis of single-stranded DNA by several different DNases (DNase II, KB DNase, S1 nuclease) under a variety of reaction conditions, but it has no effect on DNase I-catalyzed hydrolysis of single-stranded DNA. The DBP also inhibits the rate of hydrolysis of double-stranded DNA by KB DNase and DNase II, but has no effect on DNase I-catalyzed hydrolysis of this substrate. The DBP also inhibits the dephosphorylation of 5'-phosphoryl-terminated DNA by bacterial alkaline phosphatase but stimulates the phosphorylation of 5'-hydroxyl-terminated DNA by polynucleotide kinase.
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PMID:DNase inhibition by the adenovirus DNA-binding protein exhibits specificity for the enzyme but not for the secondary structure of the DNA. 630 53