Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.22.1 (
DNase II
)
429
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Deoxyribonuclease II (
DNase II
) is a key enzyme in the phagocytic digestion of DNA from apoptotic nuclei. To understand the molecular properties of
DNase II
, particularly the processing, we prepared a polyclonal antibody against carboxyl-terminal sequences of mouse
DNase II
. In the present study, partial purification of
DNase II
using Con A Sepharose enabled the detection of endogenous
DNase II
by Western blotting. It was interesting that two forms of endogenous
DNase II
were detected--a 30 kDa form and a 23 kDa form. Neither of those forms carried the expected molecular weight of 45 kDa. Subcellular fractionation showed that the 23 kDa and 30 kDa proteins were localized in lysosomes. The processing of
DNase II
in vivo was also greatly altered in the liver of mice lacking
cathepsin L
.
DNase II
that was extracellularly secreted from cells overexpressing
DNase II
was detected as a pro-form, which was activated under acidic conditions. These results indicate that
DNase II
is processed and activated in lysosomes, while
cathepsin L
is involved in the processing of the enzyme.
...
PMID:Biogenesis and proteolytic processing of lysosomal DNase II. 2351 7
