Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.22.1 (DNase II)
 429 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Bovine serum albumin was found to have an inhibitory effect on acid DNase from rat small intestinal mucosa. The inhibitory activity showed pH-dependency. Thus, the highest inhibition was observed at about pH 4.3 but conversely the enzyme was activated at about pH 4.7. The inhibitory effect was heat-inactivated most strongly at about pH 5, but at more acidic or alkaline pHs, no inactivation was observed. Inhibitory activities of serum albumin of various species were comparable with that of bovine serum albumin. Acid DNases from guinea pig kidney and small intestinal mucosa and from rat spleen and kidney were similarly inhibited by the albumin. The acid DNase displays typical Michaelis-Menten kinetics but the kinetics became sigmoidal in the presence of the inhibitor. With increasing inhibitor concentration, the sigmoidal shape became more pronounced, and at high concentration, the DNA was able to compete with the inhibitor and to reverse its action. Among the cyanogen bromide-cleaved fragments of bovine serum albumin, fragment C (derived from the carboxyl-terminal two-thirds of the albumin) had an inhibitory effect comparable to that of intact bovine albumin, but fragment N (derived from the amino-terminal one-third of the albumin) had no activity. Reduced fragment C showed a markedly decreased effect and lost the activity completely after separation into its three component peptides. Acetylation of bovine serum albumin completely destroyed its inhibitory activity.
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PMID:Inhibitory effect of bovine serum albumin on acid deoxyribonuclease from rat small intestinal mucosa. 663 Jan 63

We have examined the effect of chloroquine on rat liver lysosomal enzyme distributions after isopycnic centrifugation in a sucrose gradient. Chloroquine injection causes the large majority of cathepsin C, acid phosphatase and N acetyl glucosaminidase to migrate towards lower density regions; on the other hand only about 50% of arylsulfatase and acid deoxyribonuclease are subjected to such a density shift. To specifically mark hepatocyte lysosomes and sinusoidal cell lysosomes, rats were injected with galactosylated bovine serum albumin (A) or mannosylated bovine serum albumin (B) labelled with 125I tyramine cellobiose; A is selectively endocytosed by hepatocytes, B by sinusoidal cells. The radioactivity distribution is affected by chloroquine in the same way as cathepsin C, after injection of A though it is not influenced by chloroquine after the injection of B. These results show that chloroquine does not modify the density of liver sinusoidal cell lysosomes when it decreases the density of hepatocyte lysosomes. Such a difference could result from the fact that sinusoidal cell lysosomes do not accumulate chloroquine to the same extent as hepatocyte lysosomes. Chloroquine treatment could be useful to distinguish between hepatocyte lysosomes and sinusoidal cell lysosomes.
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PMID:Chloroquine allows to distinguish between hepatocyte lysosomes and sinusoidal cell lysosomes. 843 31