Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.22.1 (
DNase II
)
429
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The degree of 5-methylcytosine formation in DNA sequences differing in reassociation rate and susceptibility to
DNase II
digestion has been investigated in human chronic myelogenic leukemia and
acute leukemia
leukocytes, human PHA-stimulated lymphocytes and murine L5178Y lymphoblasts cultured in various phases of growth. The results indicate that in all forms of cells studied by us the general pattern of intragenomic 5-methylcytosine distribution is similar, with two preferentially methylated regions: the sequences fast reassociating and rendered Mg++-soluble after
DNase II
digestion of nuclei. The most variable fraction as regards the level of methylation seemed to be DNA of Mg++-soluble fraction of
DNase II
digest, which in
acute leukemia
leukocytes, PHA-stimulated lymphocytes and exponentially growing L5178Y cells revealed about twice greater relative proportion of methylated cytosines than in leukocytes of chronic myelogenic leukemia and L5178Y cells maintained at saturation density.
...
PMID:Intragenomic distribution of 5-methylcytosine in various forms of human and murine leukemic cells. 739 57
