Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.21.1 (DNase)
 7,655 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In 1983 the vernacular name Enteric Group 501 was coined for a group of strains that had been referred to our laboratory as "possible Vibrio damsela that does not require NaCl for growth." By DNA-DNA hybridization (hydroxyapatite method, 32P, 60 and 75 degrees C), six strains of Enteric Group 501 were closely related to the labeled strain 2446-81 (70 to 95% at 60 degrees C and 71 to 93% at 75 degrees C; 0 to 1% divergence). Type strains of all Aeromonas species and reference strains of six other Aeromonas DNA hybridization groups were 26 to 42% related (60 degrees C) to strain 2446-81, but type strains of 27 Vibrio and Photobacterium species, including V. damsela, were 0 to 1% (75 degrees C) related. We propose the name Aeromonas schubertii for the highly related group of seven strains formerly known as Enteric Group 501. The type strain is designated as ATCC 43700 (CDC 2446-81). Strains of A. schubertii grew well at 36 degrees C and had positive reactions at this temperature for methyl red, Voges-Proskauer (1% NaCl, Coblentz method), lysine decarboxylase, arginine dihydrolase, motility, lipase, DNase, nitrate reduction to nitrite, oxidase, and growth in nutrient broth with 0 and 1% NaCl. There was no growth in 6% NaCl or on thiosulfate-citrate-bile salts-sucrose agar. The following sugars were fermented: D-glucose, D-galactose, maltose, D-mannose, and trehalose. The following sugars were not fermented: adonitol, L-arabinose, D-arabitol, cellobiose, dulcitol, erythritol, myo-inositol, lactose, D-mannitol, melibiose, alpha-CH3-D-glucoside, raffinose, L-rhamnose, salicin, D-sorbitol, sucrose, and D-xylose. Esculin was not hydrolyzed, and the string test was negative. The mannitol-negative reaction differtiates A. schubertii from other Aeromonas species. The antibiogram of this organism is typical of other Aeromonas strains (resistance to ampicillin and carbenicillin and susceptibility to most other agents). A. schubertii strains have been isolated from abscesses (two strains), wound (one), skin (one), pleural fluid (one), and blood (two). The two blood isolates suggest clinical significance typical of other Aeromonas species , but further information is needed on this group.
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PMID:Aeromonas schubertii, a new mannitol-negative species found in human clinical specimens. 313 6

Ten Basidiobolus ranarum (= Basidiobolus haptosporus) strains, isolated from faeces of 102 different lower vertebrates (ectotherms) exhibited in Antwerp Zoo, or from their environment were studied for their temperature requirements, haemolysis and other enzyme activities in vitro. All isolates grew well at 25 and 37 degrees C. Three strains that produced undulated zygospore walls were haemolytic and positive for hyaluronidase. All the isolates produced urease, N-acetyl-beta-glucosaminidase, trypsin, lipase, lecithinase, gelatinase, collagenase and elastase, but failed to produce amylase, keratinase and beta-glucosidase. Three isolates failed to produce phosphatase. Only one strain failed to produce DNase. Aesculin was not hydrolysed. Chitinase activity was inconclusive. The results of this study illustrate the importance of exotic animals kept in temperate regions as carriers of potentially pathogenic organisms. In addition to the morphological characteristics, the identification can be based on enzymatic profiles. Enzymatic activity detection may help to explain the pathogenic mechanism of the fungus.
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PMID:Isolation of Basidiobolus ranarum from ectotherms in Antwerp zoo with special reference to characterization of the isolated strains. 1042 99