Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.21.1 (DNase)
 7,655 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A flow cytometric procedure measuring the 5-bromo-2-deoxyuridine incorporated into the DNA of cells in S phase was modified to make it compatible with the immunofluorescence labelling of cell surface antigens. The modifications were introduced at the stages of cell fixation and DNA denaturation. Ethanol was replaced by Tween 20-containing paraformaldehyde and hydrochloric acid was used for the denaturation of DNA by bovine pancreatic DNase-I. These two modifications permitted the preservation of immunofluorescence properties and the use of fluorochromes of the phycobiliprotein family such as phycoerythrin and allophycocyanin. This new procedure is suitable for evaluating leucocyte subsets proliferating in vitro following stimulation. As an illustration the immunosuppressive effect of cyclosporin A on PHA stimulated T4-lymphocytes was evaluated.
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PMID:Identification of DNA-replicating lymphocyte subsets using a new method to label the bromo-deoxyuridine incorporated into the DNA. 134 75

Effect of alcohol consumption on the activity of two lysosomal nucleases, deoxyribonuclease II (DNAase II) and ribonuclease II (RNAase II) and calcium concentration have been studied during liver regeneration of Sprague-Dawley rats over a period of 10 days following 70% partial hepatectomy. Liver weight was completely restored in partially hepatectomized rats at 8 days in both sexes, but ethanol treatment resulted in only a partial restoration of liver weight at 10 days. Specific activity of DNAase II in partial hepatectomized animals increased by 50-75% at 6-12 hrs above sham operated controls, and the specific activity of RNAase II increased 2.3 fold at 6 hr, while calcium concentration decreased by 50% at 6-12 hrs. Ethanol treatment masked and/or delayed the increase in the specific activity of both enzymes at early stages of liver regeneration and also masked the decrease in calcium concentration. These results indicate that ethanol consumption delays the process of liver regeneration by altering the activity of lysosomal nucleases and calcium concentration.
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PMID:Effect of acute ethanol consumption on hepatic lysosomal enzymes and calcium concentration during rat liver regeneration. 208 76

We present evidence that ethanol alters intracellular poly(adenosine diphosphoribose) metabolism and we further describe the mechanism by which ethanol exerts its effect on polymer synthesis. One percent ethanol stimulates polymer accumulation as much as 2.5-fold but does not alter polymer degradation in intact cells following DNA damage. Ethanol directly stimulates polymer synthesis following low doses of DNA damage induce by deoxyribonuclease I in a nucleotide-permeable cell system that does not possess a functional polymer turnover system. Ethanol has no measurable effect on polymer synthesis in undamaged nucleotide-permeable cells or in permeable cells treated with high doses of deoxyribonuclease I. Ethanol concentrations that stimulate poly(adenosine diphosphoribose) polymerase activity in vitro specifically lower KDNA without affecting KNAD or Vmax. The results clearly show that ethanol alters the binding of this enzyme to the DNA component of chromatin and that this altered binding is responsible for the activation of the enzyme. Altered affinity of poly(adenosine diphosphoribose) polymerase and perhaps other regulatory proteins for chromatin may play an important role in the pathology of alcohol.
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PMID:Alteration of poly(adenosine diphosphoribose) metabolism by ethanol: mechanism of action. 310 59