Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.13.1 (
exoribonuclease
)
732
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The RNA exosome complex plays a central role in RNA processing and regulated turnover. Present both in cytoplasm and nucleus, the exosome functions through associations with ribonucleases and various adapter proteins (reviewed in [Kilchert
et al
., 2016]). The RNA exosome-associated EXOSC10 protein is a distributive, 3'-5'
exoribonuclease
. The following protocol describes an approach to monitor the ribonucleolytic activity of affinity-purified EXOSC10-containing RNA exosomes, originating from
HEK
-293 cells, as reported in (Domanski
et al
., 2016) and further detailed in the companion bio-protocol to this one (Domanski and LaCava, 2017).
...
PMID:RNA Degradation Assay Using RNA Exosome Complexes, Affinity-purified from HEK-293 Cells. 2867 Jun 4
