Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.13.1 (
exoribonuclease
)
732
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
To study the physiological significance of adenosine 3'-monophosphate (
3'-AMP
), an intracellular P-site inhibitor of adenylate cyclase, in rat liver mitochondria, a specific, rapid and reliable assay method for determination of
3'-AMP
and the activity of its forming enzyme is required.
3'-AMP
in rat liver was determined to be ca. 23+/-7 nmol/g wet weight, but no 2-deoxy-
3'-AMP
, another P-site inhibitor of adenylate cyclase, was detected, even when using a reversed-phase HPLC column with a fluorescent-reaction, as established in this study. By using the optimized assay method developed here,
3'-AMP
forming enzyme activity in rat crude mitochondrial extract was found to be enhanced by EDTA and inhibited by p-chloromercurybenzoate. The optimum pH was ca. 5.8 and no divalent cation was required for activity. From these results,
3'-AMP
forming enzyme(s) in rat liver mitochondria could be classified as acid
exoribonuclease
, which mainly existed in an active form. The results obtained in this study will help to gain more insight into the physiological roles of
3'-AMP
in living systems.
...
PMID:A specific and rapid method for determination of adenosine 3'-monophosphate (3'-AMP) content and 3'-AMP forming enzyme activity in rat liver mitochondria, using reversed-phase HPLC with fluorescence detection. 988 52
