Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.13.1 (
exoribonuclease
)
732
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
T. brucei cytoplasmic calcium-dependent alkaline ribonuclease activity from DEAE-cellulose fractionation was separated into endoribonuclease and
exoribonuclease
activities by hydroxyapatite chromatography. T. brucei cytoplasmic extract markedly decreased the endoribonuclease activity, but slightly potentiated the activities of the
exoribonuclease
and bovine ribonuclease A. While the endoribonuclease was activated by
trypsin
, the
exoribonuclease
and bovine ribonuclease A were partially inactivated by
trypsin
. The endoribonuclease was activated by p-chloromercuribenzoate or N-ethylmaleimide; the
exoribonuclease
was not affected by these sulfhydryl group reagents. Free ribonuclease was separated from the latent endoribonuclease by 1 M NaCl-Sephadex G-100 gel filtration. The results demonstrate that T. brucei cytoplasm contains a latent endoribonuclease consisting of ribonuclease and inhibitor protein.
...
PMID:Trypanosoma brucei: calcium-dependent endoribonuclease is associated with inhibitor protein. 222 4
