Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.1.8 (cholinesterase)
 12,691 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We describe a method for measuring plasma cholinesterase (EC 3.1.1.8) and dibucaine and fluoride numbers by using the Cobas-Bio. Benzoyl choline chloride is used as substrate. Reaction conditions are the same as in the corresponding manual method. The reaction is stopped with physostigmine. Choline oxidase (EC 1.1.3.17) is coupled with 4-aminoantipyrene. In the presence of peroxidase (EC 1.11.1.7), the 2-hydroxy-3,5-dichlorobenzenesulfonate indicator reaction gives a red product, measured at 505 nm. The analyzer is used in the "Multi Run" mode, with plasma cholinesterase and dibucaine and fluoride inhibition concurrently measured for eight samples. Coefficients of correlation between the manual and present method for plasma cholinesterase and dibucaine and fluoride numbers in 40 patients of various phenotypes were 0.843, 0.923, and 0.717, respectively. The inter-batch CV for each of the three assays was 2% to 3%.
 ...
PMID:Cholinesterase assay automated in the Cobas-Bio centrifugal analyzer. 360 67

Choline oxidase and cholinesterase were found to retain their activity for 1-2 weeks at room temperature while adsorbed to a commercially available anion-exchange cartridge. These enzymes convert acetylcholine to H2O2. Acetylcholine can be measured in tissue extracts by separation at pH 7 on a polymeric reverse-phase high-performance liquid chromatography column, conversion of acetylcholine to H2O2 on a postcolumn enzyme-loaded anion-exchange cartridge, and electrochemical detection of the H2O2 formed.
 ...
PMID:Acetylcholine measurement by high-performance liquid chromatography using an enzyme-loaded postcolumn reactor. 639 98

The authors study 14 different analytical parameters in the pleural fluid in order to recognize differential biological criteria, helping to establish an etiologic diagnosis in patients with suggestive clinical symptoms and biological data of an infectious process. In a group of 38 patients with bacterial exudative pleural effusion (22 of tuberculous origin and 16 secondary to non-specific bacterial infection), the following parameters were analyzed: total proteins, acid glucoprotein, X1, antytripsin, CDH, acid phosphatase, amylase, cholinest, copper, iron, pCO2, pO2 pH, glucose, and cholesterol. The results of amylase, copper, pCO2, pO2 and pH determinations in the pleural fluid show statistical significant differences between the tuberculous cases and the patients with non-specific infections. Lastly, the authors mention the minimal biological criteria necessary to confirm the tuberculous or non-specific bacterial etiology of a pleural fluid, stressing the value of the levels of cholinesterase, copper, pO2 and pH as differential data.
 ...
PMID:[Tuberculous pleural effusion and pleural effusion secondary to non-specific bacterial infection: biochemical differential diagnosis (author's transl)]. 736 79