Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.1.8 (cholinesterase)
 12,691 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Several reaction principles can be used for the detection of organophosphorous compounds. E.g. the "molybdenum blue" method, the colorimetric and fluorimetric variants of the Schoenemann reaction, electrochemical detection of cyanides upon reaction with certain oximes, photoemission of exitated HPO molecules in a hydrogen rich flame and the well known enzymatic detection of cholinesterase inhibiting compounds. Various detection systems have been developed varying from simple, manually operated devices to more sophisticated and continuous functioning detectors. These well established detection principles and some new emerging techniques, like microsensors using the surface acoustic wave technique, will be discussed.
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PMID:Various means for the detection of organophosphorous compounds. 357 Jun 3

Aerotoxic syndrome is assumed to be caused by exposure to tricresyl phosphate, an additive in engine lubricants and hydraulic fluids that is activated to the toxic 2-(ortho-cresyl)-4H-1,3,2-benzodioxaphosphoran-2-one (CBDP). Currently, there is no laboratory evidence to support intoxication of airline crew members by CBDP. Our goal was to develop methods for testing in vivo exposure by identifying and characterizing biomarkers. Mass spectrometry was used to study the reaction of CBDP with human albumin, free tyrosine, and human butyrylcholinesterase. Human albumin made a covalent bond with CBDP, adding a mass of 170amu to Tyr411 to yield the o-cresyl phosphotyrosine derivative. Human butyrylcholinesterase made a covalent bond with CBDP on Ser198 to yield five adducts with added masses of 80, 108, 156, 170, and 186amu. The most abundant adduct had an added mass of 80amu from phosphate (HPO(3)), a surprising result given that no pesticide or nerve agent is known to yield phosphorylated serine with an added mass of 80amu. The next most abundant adduct had an added mass of 170amu to form o-cresyl phosphoserine. It is concluded that toxic gases or oil mists in cabin air may form adducts on plasma butyrylcholinesterase and albumin, detectable by mass spectrometry.
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PMID:Development of diagnostics in the search for an explanation of aerotoxic syndrome. 2044 73