Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.1.8 (
cholinesterase
)
12,691
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Acetylthiocholine iodide
(
ATC
) as a common substrate in the combined assay of red blood cell
cholinesterase
(RBC ChE) and
butyrylcholinesterase
(BuChE) do not provide the accurate individual enzyme activities. Hence, in the present study the two enzyme activities in the same sample were assayed with the help of two different substrate,
ATC
and butyrylthiocholine iodide (BTC). Specificity of BTC towards BuCHE was found in blood, plasma and serum, while
ATC
is nonspecifically hydrolysed by both RBC ChE and BuChE.
ATC
gives significantly higher enzyme activity (P < 0.001) in rat plasma/serum and significantly lower enzyme activity (P < 0.0001; P < 0.001) in human plasma/serum. The possible reasons are discussed for substrate specity in various species in the assay of ChEs.
...
PMID:Species variation in the specificity of cholinesterases in human and rat blood samples. 781 85
A modified colorimetric method for the estimation of
cholinesterase
activity has been worked out using two different substrates, acetylthiocholine iodide for total
cholinesterase
and a specific substrate, butyrylthiocholine iodide for
pseudocholinesterase
in the same sample. This is a modification of the method described by Voss and Sachsse (1970) wherein acetylthiocholine iodide was used for both total and pseudo
cholinesterase
activities. The
pseudocholinesterase
obtained with acetylthiocholine iodide was significantly higher (P < 0.0001) than that with butyrylthiocholine iodide either in whole blood or serum samples.
Acetylthiocholine iodide
while reacting with
pseudocholinesterase
in serum or plasma samples might also be interacting with the small quantities of acetylcholinesterase present. It is therefore suggested that butyrylthiocholine iodide and acetylthiocholine iodide may be used to determine
pseudocholinesterase
and total
cholinesterase
activities respectively. The use of two substrates with a few more alterations in the experimental conditions increased the validity of this simple and rapid colorimetric method.
...
PMID:Improved colorimetric method for cholinesterase activity. 844 54
