Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.1.79 (
hormone-sensitive lipase
)
2,163
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Okadaic acid
was found to induce concentration- and time-dependent lipolysis in rat fat cells in the absence of lipolytic hormones, but it did not significantly increase the total
hormone-sensitive lipase
(
HSL
) activity in these fat cells, the activity of
HSL
extracted from fat layer and that of
HSL
in the supernatant of homogenized fat cells. Western blotting of fat cell homogenate fractions with an antiserum raised against synthetic peptide derived from rat
HSL
showed that
HSL
protein shifted from the supernatant to the fat layer in response to okadaic acid, which increased the
HSL
protein content on the fat layer and concomitantly reduced that of the supernatant, concentration- and time-dependently. Sonication of the fat cells abolished their responsiveness to okadaic acid. The lipolytic action of okadaic acid was examined and its site was identified using a cell-free system comprising lipid droplets isolated from rat fat cells and
HSL
.
Okadaic acid
induced lipolysis in this cell-free system and sonication of the lipid droplets caused disappearance of lipolytic action of okadaic acid.
Okadaic acid
failed to stimulate lipolysis in a cell-free system comprising
HSL
and artificial lipid droplets (trioleoylglycerol emulsified with gum arabic) instead of lipid droplets isolated from rat fat cells. These results suggest that okadaic acid does not increase the catalytic activity of
HSL
but induces translocation of
HSL
to the lipid droplets isolated from rat fat cells. The site of the lipolytic action of okadaic acid in relation to the interaction between
HSL
and lipid droplet is discussed.
...
PMID:Mechanism of the stimulatory action of okadaic acid on lipolysis in rat fat cells. 1068 3
