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Query: EC:3.1.1.79 (
hormone-sensitive lipase
)
2,163
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Numerous gram-negative bacteria have quorum-sensing systems and produce AHL as a quorum-sensing signal molecule. In this study, we demonstrated that
Methylobacterium populi
P-1M, an isolate from a pink-pigmented household biofilm, produced two AHLs, C14:1-
HSL
as a predominant product and 3OHC14-
HSL
as a minor product. The complete genome sequence of
M. populi
P-1M revealed the presence of genes that are predicted to encode an AHL synthase (
mpoI
) and AHL receptor (
mpoR
).
M. populi
P-1M formed a pellicle-like biofilm, which had a flat surface and was easily removable. In contrast, biofilms formed by
mpoI
and/or
mpoR
deletion mutants had a wavy surface structure and strongly adhered to the glass tube. When C14:1-
HSL
was added to the
mpoI
mutant culture, the biofilm structure resembled that of the wild-type strain. These results demonstrated that the structure and adhesion strength of
M. populi
P-1M biofilms are determined in part by AHL-mediated quorum sensing.
Abbreviations:
AHL:
N
-acyl-l-homoserine lactone; C14:1-
HSL
:
N
-tetradecenoyl-l-homoserine lactone; 3OHC14-
HSL
:
N
-(3-hydroxytetradecanoyl)-l-homoserine lactone; SAM:
S
-adenosyl-l-methionine;
ACP
: acyl-acyl carrier protein; EPS: extracellular polysaccharide; DMSO: dimethyl sulfoxide.
...
PMID:
N
-Acylhomoserine lactone-mediated quorum sensing regulates biofilm structure in
Methylobacterium populi
P-1M, an isolate from a pink-pigmented household biofilm. 3020 Aug 29
