Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.1.79 (
hormone-sensitive lipase
)
2,163
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The effects of
2,5-piperazinedione
in reducing the production of quorum sensing (QS)-dependent factors in Pseudomonas aeruginosa PAO1 were assessed both in vitro and in vivo.
2,5-Piperazinedione
exhibited a 69% reduction in the azocasein-degrading proteolytic activity and a 48% reduction in the elastolytic activity of PAO1. Further, it showed 85% and 96% reduction in the production of pyocyanin and extracellular polymeric substances (EPS) of PAO1, respectively. In the swimming inhibition assay,
2,5-piperazinedione
-treated PAO1 cells exhibited poor swimming motility in swim agar medium. In the in vivo analysis, an enhanced survival of PAO1-preinfected Caenorhabditis elegans was observed after treatment with
2,5-piperazinedione
. Regarding the mode of action, in the molecular docking analysis,
2,5-piperazinedione
interacts with the amino acid residue of the LasR receptor protein required for binding the natural ligand N -3-oxododecanoyl-l-homoserine lactone (3-oxo-C12-
HSL
). This demonstrates the probability of
2,5-piperazinedione
to interfere with the binding process of 3-oxo-C12-
HSL
to its receptor protein. Thus, the findings of the present study reveal the potential of
2,5-piperazinedione
in reducing the QS-dependent phenotypic features of PAO1.
...
PMID:2,5-Piperazinedione inhibits quorum sensing-dependent factor production in Pseudomonas aeruginosa PAO1. 2235 66
