Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.1.79 (
hormone-sensitive lipase
)
2,163
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Antisense long non-coding RNAs (AS lncRNAs) play important roles in refined regulation of animal gene expression. However, their functions and molecular mechanisms for domestic animal adipogenesis are largely unknown. Here, we found a novel AS lncRNA transcribed from the porcine
PU.1
gene (also known as SPI1) by strand-specific RT-PCR. Results showed that
PU.1
AS lncRNA was expressed and generally lower than the level of
PU.1
mRNA in porcine subcutaneous adipose, heart, liver, spleen, lympha, skeletal muscle and kidney tissues. We further found that the levels of
PU.1
mRNA and
PU.1
protein were significantly lower in subcutaneous and intermuscular adipose than in mesenteric and greater omentum adipose, whereas the levels of
PU.1
AS lncRNA showed no difference in porcine adipose tissues from four different parts of the body. During porcine adipogenesis, levels of
PU.1
mRNA increased at day 2 and then gradually decreased. Meanwhile,
PU.1
AS lncRNA exhibited an expression trend similar to
PU.1
mRNA but sharply decreased after day 2. Interestingly,
PU.1
protein level rose during differentiation. In addition, at day 6 after differentiation, knockdown of endogenous
PU.1
promoted adipogenesis, whereas knockdown of endogenous
PU.1
AS lncRNA had the opposite effect. Moreover, peroxisome proliferator-activated receptor gamma (PPARG) and fatty acid synthase (FASN) were significantly upregulated in the
PU.1
shRNA treatment group (P < 0.05), whereas they were downregulated in the
PU.1
AS shRNA treatment group (P < 0.05). Adipose triglyceride lipase [ATGL; also known as patatin-like phospholipase domain containing 2 (PNPLA2)] and
hormone-sensitive lipase
[
HSL
; also known as lipase, hormone-sensitive (LIPE)] contrasted with PPARG and FASN. Finally, the
PU.1
mRNA/
PU.1
AS lncRNA duplex was detected by an endogenous ribonuclease protection assay combined with RT-PCR. Based on the above results, we suggest that
PU.1
AS lncRNA (vs. its mRNA translation) promotes adipogenesis through the formation of a sense-antisense RNA duplex with
PU.1
mRNA.
...
PMID:PU.1 antisense lncRNA against its mRNA translation promotes adipogenesis in porcine preadipocytes. 2569 Nov 51
