Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:3.1.1.7 (acetylcholinesterase)
28,390 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A new enzymatic method for the determination of serum pseudo-cholinesterase activity is described. Choline, which is liberated from benzoylcholine as substrate by cholinesterase, is oxidized by choline oxidase to betaine with the simultaneous production of hydrogen peroxide, which oxidatively couples with 4-aminoantipyrine and phenol in the presence of peroxidase to yield a chromogen with maximal absorbance at 500 nm. The calibration curve is linear up to 1500 units per liter of serum. The method is reproducible, and the results correlate well with those obtained by the method using butyrylthiocholine as substrate and 5,5'-dithiobis-(2-nitrobenzoic acid) as color reagent.
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PMID:New enzymatic assay of cholinesterase activity. 2 Feb 53

We investigated Gl. submandibularis and Gl. sublingualis of the guinea-pig 1, 2, 5, 7, 14 und 28 days after section of the Chorda tympani with histological-histochemical methods. The innervation pattern of both glands (Gl. submandibularis: aminergic-cholinergic double innervated; Gl. sublingualis: cholinergic innervated) remains unchanged. In the gland cells the following effects were observed: a) Gl. sublingualis. In the first 3 days apocrine and holocrine secretion phenomena are often seen, suggesting a maximal stimulation of the gland parenchyma. They are accompanied with cellular reactions of the interstitial space. In a second phase a new gland cell population appears that uniformly exhibits intracellular accumulation of secretion products. Involution begins from the 14th day on. Secretory cells are dedifferentiated to intercalated duct cells; autophagic processes help to degradate the accumulated secretion granules. b) Gl. submandibularis. Here the effects are less dramatic. The accumulation of the secretory granules starts as soon as 24 h after section of the Chorda and is maximal between the 5th and 8th p. o. day. Involution of the gland begins from the 14th day on. The accumulated secretory granules show high activities of two histochemically demonstrable enzymes, the cholinesterase and the peroxidase.
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PMID:[Histologic-histochemical findings in the salivary glands of guinea pigs after sectioning the chorda tympani]. 4 27

The axoplasmic retrograde transport of horseradish peroxidase (HRP) from axon terminals to their parent cell bodies and histochemical fluorescence microscopy have been used to study the ipsilateral centrifugal fibers to the olfactory bulbs and anterior olfactory nucleus in the rabbit. Focal injections of peroxidase were placed unilaterally into the main or accessory olfactory bulb or into the anterior olfactory nucleus. In animals with injected HRP confined within the main bulb, perikarya retrogradely labeled with the protein in the ipsilateral forebrain were observed in the anterior prepyriform cortex horizontal limb of the nucleus of the diagonal band, and far lateral preoptic and rostral lateral hypothalamic areas. Brain stem cell groups that contained HRP-positive somata include the locus coeruleus and midbrain dorsal raphe nucleus. Except for the prepyriform cortex, the basal forebrain structures with labeled perikarya correlate well with locations of cell bodies containing acetylcholinesterase and choline acetyltransferase. These somata may represent a cholinergic afferent system to the main olfactory bulb. Peroxidase-labeled cell bodies in the locus coeruleus and midbrain raphe are indicative of noradrenergic and serotonergic innervations respectively of the olfactory bulb. In rabbits in which peroxidase was injected or diffused into the accessory olfactory bulb and anterior alfactory nucleus, HRP-positive somata were identified in the prepyriform cortex bilaterally, the horizontal limb of the diagonal band nucleus, lateral hypothalamic region, nucleus of the lateral olfactory tract, corticomedial complex of the amygdala, mitral and tufted cell layers of the ipsilateral main olfactory bulb, locus coeruleus, and the midbrain raphe. Evidence for centrifugal fibers to the accessory olfactory bulb from the corticomedial complex of the amygdala, locus coeruleus, and possibly the nucleus of the lateral olfactory tract and midbrain raphe is discussed. A similar distribution of labeled perikarya in the forebrain and brain stem was seen in rats in which peroxidase injected into the main olfactory bulb had spread into the accessory bulb and anterior olfactory nucleus. Histochemical fluorescence microscopy of the main and accessory olfactory bulbs in the rabbit and rat revealed fine caliber, green fluorescent fibers and varicosities predominantly in the granule cell layer and less so among cells in the glomerular layer. In sections through the root of the main olfactory bulb, a similar fluorescence was seen in the deep half of the plexiform layer of the pars externa of the anterior alfactory nucleus. These fluorescent fibers likely represent the noradrenergic innervation of the olfactory bulbar and retrobulbar formations. A fluorescent yellow hue was observed in the glomerular layer of the main bulb and may signify a serotonergic innervation of this lamina...
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PMID:Olfactory relationships of the telencephalon and diencephalon in the rabbit. III. The ipsilateral centrifugal fibers to the olfactory bulbar and retrobulbar formations. 6 70

The origins, distribution, and cellular targets of the septo-hippocampal projections are reviewed. It appears that the distribution of acetylcholinesterase-positive neurons in the medial septum and diagonal bands and those cells labelled after injections of horseradish peroxidase into the hippocampus coincide; however, the possibility of a non-acetylcholinesterase septal projection remains. Good agreement is found between the distribution of hippocampal acetylcholinesterase and the patterning of silver grains after injection of [3H]leucine into the medial septum. A major target of septal efferents to the hippocampus is the interneuron population; the possibility of septal mediation of intrahippocampal circuitry via this anatomical arrangement is discussed.
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PMID:Anatomical and functional aspects of the septo-hippocampal projections. 8 25

We examined the role of nerve terminals in organizing acetylcholine receptors on regenerating skeletal-muscle fibers. When muscle fibers are damaged, they degenerate and are phagocytized, but their basal lamina sheaths survive. New myofibers form within the original basal lamina sheaths, and they become innervated precisely at the original synaptic sites on the sheaths. After denervating and damaging muscle, we allowed myofibers to regenerate but deliberately prevented reinnervation. The distribution of acetylcholine receptors on regenerating myofibers was determined by histological methods, using [125I] alpha-bungarotoxin or horseradish peroxidase-alpha-bungarotoxin; original synaptic sites on the basal lamina sheaths were marked by cholinesterase stain. By one month after damage to the muscle, the new myofibers have accumulations of acetylcholine receptors that are selectively localized to the original synaptic sites. The density of the receptors at these sites is the same as at normal neuromuscular junctions. Folds in the myofiber surface resembling junctional folds at normal neuromuscular junctions also occur at original synaptic sites in the absence of nerve terminals. Our results demonstrate that the biochemical and structural organization of the subsynaptic membrane in regenerating muscle is directed by structures that remain at synaptic sites after removal of the nerve.
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PMID:Acetylcholine receptors in regenerating muscle accumulate at original synaptic sites in the absence of the nerve. 47 8

The areas surrounding the fornix longus were examined using the rapid Golgi method, acetylcholinesterase (AChE) histochemistry, and horseradish peroxidase histochemistry. Parasagittal Golgi impregnated sections revealed the neuronal configurations of the nuclei of the fornix longus. An interstitial nucleus within the fiber bundle and a bed nucleus were observed. They coursed from midline dorsal hippocampus and became continuous with caudal septal regions (notably septotriangularis). Neurons in the interstitial nucleus were spine-poor whereas bed nucleus neurons were spiny. Numerous axon collaterals were observed in the area. These gave rise to many apparent synaptic boutons. This intrinsic connectivity within the fornix longus was verified physiologically. Acetylcholinesterase histochemistry revealed the presence of AChE positive fibers traveling in both the bed nucleus and fornix longus. These fibers appeared to emanate from the regions of the diagonal band and formed a marked density in the bed nucleus. Also apparent was a striking midline density of AChE that forms a distinct midline fornix. All of these AChE positive fibers appeared to be related to the medial portions of the dorsal hippocampus from its septal pole to the dorsal psalterium. Injections of horseradish peroxidase into medial septal regions resulted in pick-up of the peroxidase in a specialized portion of hippocampus. Specifically, neurons were identified in the alvear portions of the midline dorsal hippocampus as well as in the alveus and stratum oriens (occasionally) of CA 3. It was concluded that the area of the fornix longus is situated in the mainstream of many incoming and outgoing hippocampal fiber systems. Because of this unique situation and the bi-directionality of the projections fibers of the area, the bed nucleus and interstitial nucleus of the fornix longus were postulated to play a crucial role in normal hippocampal processing.
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PMID:The regions of the fornix longus: a morphologic analysis. 53 86

The effects of bilateral injections of kainic acid into the anteromedial neostriatal region were examined behaviorally and anatomically in two groups of rats. Behaviorally, kainic acid injections resulted in a severe impairment of delayed alternation retention, while the ability for visual discrimination remained unaffected. Anatomically it was found that axons traversing the injected area remain able to transport horseradish peroxidase. Furthermore, histological examinations of the injected regions revealed a heavy loss of neurons and a decrease of histochemical staining for specific acetylcholinesterase. Silver impregnation showed slightly disorganized, but continuous, axons in bundles of the capsula interna. On the other hand, the axonal network throughout the neuropil of the injected area was markedly diminished. No conspicuous change was found in myelin staining or in the intensity or catecholamine fluorescence. The anatomical results suggest that kainic acid appears to affect only perikarya of the neostriatum and the axons originating from these perikarya, whereas passing axons seem to remain intact. Thus, the observed behavioral impairment must be attributed to changes in the neostriatum itself. It is concluded that the neostriatum has 'complex' or 'cognitive' functions and that some mental symptoms in Huntington's chorea may be attributed to a dysfunction of this part of the brain.
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PMID:Behavioral and anatomical consequences of small intrastriatal injections of kainic acid in the rat. 66 28

This paper reports a study of changes in red blood cell enzymes and some serum parameters during and after treatment of protein-calorie malnutrition. The red cell GSH levels were low during the crisis, together with the levels of GSSG:NADPH reductase, GSH:H2O2 peroxidase, aspartate aminotransferase and alanine aminotransferase. After treatment the levels of all these enzymes increased significantly to normal values. Of the serum parameters investigated, significant reduction in the activity of the enzymes cholinesterase, catecholamine oxidase, total proteins, albumin, urea and electrolytes were obvious, and returned to normal values after treatment. Ceruloplasmin activity remained low even after three weeks' treatment and could not be related to copper levels. The results are discussed in relation to anemia and liver damage that may accompany the syndrome.
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PMID:Protein-calorie malnutrition: a study of red blood cell and serum enzymes during and after crisis. 82 Apr 94

The dermal cells in grey, xanthic, and white goldfish integuments were cytochemically characterized for the following enzymatic activities: tyrosinase, DOPA-oxidase, cytochrome oxidase, monoamine oxidase, peroxidase, non-specific esterase, cholinesterase, NAD-diaphorase, NADP-diaphorase, aryl sulfatase, nucleotide phosphodiesterase, beta-glucuronidase, acid phosphatase, alkaline phosphatase, adenosine triphosphatase, thiamine pyrophosphatase, glucose-6-phosphatase, aldolase, as well as succinate, malate, isocitrate, glutamate, glucose-6-phosphate, 6-phosphogluconate, alpha-glycerophosphate, alcohol, lactate, and beta-hydroxybutyrate dehydrogenases. It was found that the epidermis was a significant barrier to the access of cytochemical reaction substrates. Removal of the epidermal barrier provided dermal cell localizations of enzymatic activities which were reproducible. Further, alterations in reaction times and temperatures from the mammalian methodology provided conditions fe various integumental cells were compared for possible interrelationships. The basic foundations for future work with the dermis of poikilothermic vertebrates on an experimental basis were established. In addition, a previously undescribed non-pigmented dermal cell, the "x"-cell, was found to have enzymatic characteristics similar to both melanophores and lipophores. The "x"-cell may be the common precursor of both types of pigment cells.
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PMID:Cytochemical characterization of goldfish (Carassius auratus L.) dermis with special reference to the pigment cells. 82 86

A new wafer embedding procedure is described that permits light microscopic screening of embedded tissue prior to ultrathin sectioning. It is particularly valuable when used on specimens obtained with an automatic sectioner and treated cytochemically to obtain visible intermediate or visible and electron opaque final reaction products. Aldehyde-fixed tissues are cut into sections with an automatic sectioner, incubated cytochemically osmication if required, then embedded in epoxy resin between fluorocarbon coverglasses which are supported by a platform specially designed for this purpose. The resultant wafter, less than 0.2 mm thick, is examined by light microscopy for optimal areas of cytochemical reaction and desirable structural features. Such areas are cut out and glued to blank blocks with fast curing cyanoacrylate cement for subsequent ultrathin sectioning. The usefulness of this technique is demonstrated by the location of: (1) esterase-positive lysosomes in kidney and trigeminal ganglia; (2) palatal sensory endings stained for acetylcholinesterase; and (3) phagosomes arising from the resorption of horseradish peroxidase tracer by the cuboidal parietal epithelial cells of Bowman's capsule in the male mouse.
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PMID:Wafer embedding: specimen selction in electron microscopic cytochemistry with osmiophilic polymers. 86 47


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