Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.1.7 (
acetylcholinesterase
)
28,390
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The article presents a novel strategy for a sensitive investigation of the interaction between
acetylcholinesterase
(
AChE
) and its small molecular carbamate inhibitors. Two carbamate inhibitors with different ether linkages and the terminal lipoate were synthesized and labeled with gold nanoparticles (AuNPs). With the signal amplification of AuNPs, the specific interactions between the AuNPs labeled carbamate inhibitors (
ALC1
and ALC2) and the immobilized
AChE
on sensor chip surface were readily examined. The detection sensitivities of
ALC1
and ALC2 were 176 and 121 m degrees /nM, respectively, with the detection limits of 7.0 and 12pM at a signal-to-noise ratio of 3. The association/dissociation constants for the binding interaction between carbamate inhibitors and
AChE
were reported for the first time. The affinity constants were estimated to be 3.13 x 10(6) and 6.39 x 10(5)M(-1) for
ALC1
and ALC2 respectively. This AuNPs labeling strategy is versatile and may be applicable for the direct or competitive SPR kinetic assay of the interaction between small molecule inhibitors and their target proteins with a high sensitivity.
...
PMID:A gold nanoparticle labeling strategy for the sensitive kinetic assay of the carbamate-acetylcholinesterase interaction by surface plasmon resonance. 1926 69
