Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:3.1.1.7 (acetylcholinesterase)
28,390 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

NADPH-diaphorase histochemistry was combined with demonstration of acetylcholinesterase and immunocytochemistry for calcitonin gene-related peptide to study esophageal innervation in the rat. Most of the myenteric neurons stained positively for NADPH-diaphorase, as did numerous varicose nerve fibers in the myenteric plexus, among striated muscle fibers, around arterial blood vessels, and in the muscularis mucosae. A majority of motor endplates (as demonstrated by acetylcholinesterase histochemistry or calcitonin gene-related peptide immunocytochemistry) were associated with fine varicose NADPH-diaphorase-positive nerve fibers. Analysis of brainstem nuclei, sensory vagal, spinal, and sympathetic ganglia in normal and neonatally capsaicin-treated rats, and comparison with anterogradely labeled vagal branchiomotor, preganglionic and sensory fibers led to the conclusion that NADPH-diaphorase-positive fibers on motor endplates originate in esophageal myenteric neurons. No association of NADPH-diaphorase-positive nerve fibers with motor endplates was found in other organs containing striated muscle. These results suggest extensive, presumably nitrergic, co-innervation of esophageal striated muscle fibers by enteric neurons. Thus, control of peristalsis in the esophagus of the rat may be more complex than hitherto assumed.
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PMID:NADPH-diaphorase-positive nerve fibers associated with motor endplates in the rat esophagus: new evidence for co-innervation of striated muscle by enteric neurons. 818 63

The distribution of nerves and mast cells was studied in the lacrimal glands of 3-5-, 14- and 24-month-old rats, using light microscopic histochemical and immunohistochemical techniques. In 14-month and, to a greater extent, in 24-month-old rats there were signs of chronic inflammation and patchy destruction of acinar, ductal and vascular tissue. The glands of the three different age groups contained acetylcholinesterase (AChE), vasoactive intestinal polypeptide (VIP)-, neuropeptide Y (NPY)-, calcitonin gene-related peptide (CGRP)-, tyrosine hydroxylase-, substance P- and the phosphoprotein B-50-immunoreactive nerves. B-50-immunoreactive nerves were distributed around acini, blood vessels and ducts, in a similar manner to VIP and AChE. Substance P- and CGRP-immunoreactive nerves were sparsely distributed in interlobular connective tissue and around ducts and blood vessels. Tyrosine hydroxylase- and NPY-containing nerves were found around blood vessels. The 3-5- and 14-month-old rats had a similar pattern of innervation, however, by 24 months there was a reduction in the number and intensity of immunoreactive nerves. The loss of nerves was particularly associated with damage to the gland. Mast cells were also found in the lacrimal, mostly associated with neurovascular tissue. These could be histochemically labelled with alcian blue/safranin or toluidine blue and were immunohistochemically labelled with histamine and serotonin. Substance P-, CGRP-, VIP- and NPY-immunoreactive nerves were found apposed to mast cells. A large increase in mast cells was observed in 24-month compared to 3-5-month-old rats and these were found throughout the acinar tissue. These results show that a decrease in innervation and also chronic inflammation, with mast cell infiltration, occurs in aged rats. These findings may be contributing factors to reduced tear output in aging.
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PMID:Innervation and mast cells of the rat exorbital lacrimal gland: the effects of age. 818 88

The distribution, number, projections and nature of ganglia and ganglionic neurons in the feline larynx were investigated morphologically. Six to eight large oval or spindle-shaped ganglia (including 50-80 ganglionic neurons per ganglion) in rostral portions of the paraglottic spaces, four to six small spindle-shaped ganglions (containing 5-25 ganglionic perikarya in each ganglion) dorsal to the posterior cricoarytenoid muscles and one to three small elliptical ganglia (having 15-25 ganglionic cells in each ganglion) around the inferior laryngeal nerves were observed in the larynx. Each ganglion was covered with a fibrous capsule and ganglionic neurons with a diameter of 25-30 microns totaled 600 to 800. Ganglionic neurons received projections from the dorsal motor nucleus of vagus, the superior cervical ganglion (SCG) and the nodose ganglion (NG) ipsilaterally. On the other hand, ganglionic neurons projected to SCG, NG, ipsilaterally and the laryngeal mucosa bilaterally with ipsilateral predominance. Ganglionic neurons showed acetylcholinesterase positive reactions, presumably parasympathetic. On immunocytochemistry, many vasoactive intestinal polypeptide (VIP)-immunoreactive (ir) neurons, and a few tyrosine hydroxylase (TH)-ir and substance P (SP)-ir cells were recognized in ganglions, but no calcitonin gene-related peptide (CGRP)-ir neurons were found. These VIP, TH, SP-ir neurons did not change after denervation of the ipsilateral superior and recurrent laryngeal nerves. Many VIP- and some TH-, SP-, CGRP-ir fibers were also observed around vessels and glandular cells. The present findings show that intra-laryngeal ganglionic neurons not only have an endogenic cholinergic nature, but are also involved in local sympathetic and sensory nervous systems.
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PMID:[Morphological study of intra-laryngeal ganglia and their neurons in the cat]. 829 67

Immunohistochemistry and cholinesterase histochemistry were used to evaluate the structure and neurotransmitter content of the ganglionated plexuses of the human, canine, and opossum (Monodelphis domestica) gallbladders. In each species, the ganglionated plexus consisted of small (mean approximately 4 neurons/ganglion), irregularly dispersed ganglia that were interconnected by bundles of nerve fibers. The density of ganglia was about ten-fold higher in the opossum than in the human or the dog. Immunostaining for choline acetyltransferase (ChAT) was accomplished in the human, dog, opossum, and the guinea pig where all neurons were found to express ChAT-immunoreactivity. In the human, immunoreactivities for vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) were the most abundant followed by substance P (SP). In the dog, immunoreactivity for galanin (GAL) was the strongest, followed closely by VIP and then by SP. NPY-immunoreactive neurons were not observed in the dog, but immunoreactive nerve fibers were seen in the perivascular plexus. In the opossum, immunoreactivity for GAL was the most intense and abundant followed by SP, which was followed by VIP. NPY-immunoreactivity in the opossum was limited to scarce perivascular nerve fibers. Immunoreactivity for calcitonin-gene-related peptide (CGRP) was not observed in neuronal somata, but CGRP/SP-immunoreactive nerve fibers were a feature of each species studied. These findings, along with previously published work on the guinea pig, indicate that it is likely that all gallbladder neurons are cholinergic, and that VIP, SP, and NPY and/or GAL are commonly expressed in gallbladder neurons.
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PMID:Structure and chemical coding of human, canine and opossum gallbladder ganglia. 862 95

Long-term (2-12 weeks) cultures of adult guinea-pig ventricular myocytes, cocultured with neurons derived from stellate or intrinsic cardiac ganglia, retain their functional properties (Horackova et al., 1993, 1994, 1995). The present study was designed to investigate the morphological and immunochemical properties of such neurons and their associated cardiomyocytes. Cultured myocytes studied by means of phalloidin-rhodamine (for F-actin) and an antibody raised against myomes revealed parallel myofibrils with striations typical of rod-shaped cardiomyocytes, even while myocytes changed from cylindrical to flattened form as they established intercellular contacts. Microtubular networks, identified by alpha-tubulin DM1A antibody, were arrayed longitudinally in myofibrils, being especially prominent during the formation of intercellular contacts between myocytes. Histochemically identified adult peripheral autonomic neurons cultured alone or with myocytes displayed a variety of shapes. alpha-Tubulin staining was associated with the somata and neurites of various-shaped neurons whether cultured alone or with myocytes. Cultured neurons derived from stellate and intrinsic cardiac ganglia also exhibited staining for the general neuronal marker PGP 9.5 (protein gene product 9.5), and for specific markers of the following neurochemicals: tyrosine hydroxylase, acetylcholinesterase, choline acetyltransferase, neuropeptide Y, vasoactive intestinal peptide, calcitonin gene-related peptide, bradykinin, oxytocin, and NADPH-diaphorase. These data indicate that: (a) adult ventricular myocytes cocultured with intrathoracic neurons retain the structural properties of adult myocytes found in vivo; (b) intrinsic cardiac and extrinsic intrathoracic neurons cultured alone or with cardiomyocytes display morphological characteristics similar to those of neurons studied in situ; (c) intrinsic cardiac and intrathoracic extracardiac neurons cultured alone or with cardiomyocytes display a variety of morphologies (unipolar, bipolar, and multipolar), larger and more multipolar neurons being present in cultures derived from stellate versus intrinsic cardiac ganglia; (d) such cultured neurons are associated with a number of neurochemicals, more than one chemical being associated with each neuron. This model presents an excellent opportunity to study the morphology of individual peripheral extracardiac and intracardiac neurons as well as their potential to produce various neurochemicals that are known to be involved in the neuromodulation of cardiomyocyte function.
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PMID:Morphological and immunohistochemical properties of primary long-term cultures of adult guinea-pig ventricular cardiomyocytes with peripheral cardiac neurons. 876 Aug 56

This work addresses the physiological regulation of the tetrameric (G4) form of acetylcholinesterase (AChe) in end-plate regions of anterior gracilis muscles from adult male Sprague-Dawley rats subjected to short-term low-intensity treadmill exercise. Experiments involved analyses of muscle AChe molecular form activities, endogenous calcitonin gene-related peptide (CGRP) levels, and the effect of exogenous CGRP on AChe forms after exercise. Animals were exercised twice per day for 1 or 2 days. Daily training sessions of 135 min (10 min of walking alternating with 5 min of resting) were separated by a 105-min resting period. Results show that exercise causes a slight decline in endogenous CGRP and a selective increase in G4 AChe that is partially reversed by treatment with exogenous CGRP. These findings indicate that CGRP influences the mechanism(s) by which G4 AChe in intact fast-twitch anterior gracilis muscles adapts to enhanced motor activity. They are also consistent with the hypothesis that, in addition to acetylcholine, neurogenic CGRP participates in the regulation of G4 AChe at the neuromuscular junction.
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PMID:Physiological regulation of G4 AChe in fast-twitch muscle: effects of exercise and CGRP. 884 28

There is increasing evidence that neuropeptides may be involved in the pathogenesis of atopic dermatitis (AD). This study examines whether neuropeptide distribution in the skin of patients with AD differs from normal controls. The distribution and density of several neuropeptides were examined in lesional and non-lesional skin of AD patients (n = 5) and in normal controls (n = 4) using indirect immunofluorescence and image analysis. Cholinergic innervation was studied using cholinesterase histochemistry. Staining with the general neuronal marker protein gene product 9 x 5 showed a subepidermal network of nerves with fibres penetrating the epidermis, and nerves around blood vessels, sweat glands and hair follicles. Image analysis of nerves around sweat glands showed a significantly higher nerve density in non-lesional compared with both normal controls and lesional skin (P < 0.05); lesional compared with control skin showed no significant difference. In the epidermis the density of nerves was not significantly greater in non-lesional compared with lesional skin and controls. Calcitonin gene-related peptide immunoreactivity was similar in all subjects except in three of the AD patients, where more nerves appeared to penetrate the epidermis. Substance P immunoreactivity in the papillary dermis was seen in all AD patients but no controls. Vasoactive intestinal polypeptide and neuropeptide Y staining were similar in all groups. Acetylcholinesterase-positive nerves were found around sweat glands in all subjects, the staining being greatest in non-lesional and least in lesional skin. Occasional nerves were seen in the papillary dermis in lesional skin of two out of the four patients. We have demonstrated quantitative differences in nerve growth in clinically normal skin of AD patients, and altered cutaneous neuropeptide expression in these patients which may contribute to the pathogenesis of AD. The cause of atopic dermatitis (AD) has not been fully established but it is believed that there is a complex interaction between genetic susceptibility, precipitating environmental factors and disordered immune responsiveness. There is increasing evidence that neuropeptides may be involved in the pathogenesis of AD. Exacerbations of the disease can be provoked by stress, scratching and sweating which may be the result of neurogenic inflammation. One of the first features of an exacerbation is flushing of the affected skin and pruritus. Several neuropeptides that have been identified in human skin are potent inducers of vasodilation and may induce pruritus. Substance P (SP), calcitonin gene-related peptide (CGRP) and vasoactive intestinal polypeptide (VIP) all cause vasodilation when injected intradermally, and SP and CGRP have been shown to be mediators of the weal and flare reaction. Spantide, a competitive antagonist of SP, has been shown to inhibit immediate and delayed-type hypersensitivity reactions. Part of these responses may be due to release of histamine and indeed elevated concentrations of histamine have been found in vivo in the skin and plasma of patients with AD. In this study the distribution and density of several neuropeptides were examined in lesional and nonlesional skin of AD patients and in normal controls using indirect immunofluorescence and image analysis. Cholinergic innervation was studied using cholinesterase histochemistry. Because many afferent fibres do not express CGRP or SP, the general neuronal marker protein gene product (PGP 9 x 5) was used to assess the overall nerve supply to the skin.
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PMID:Neuropeptides in the skin of patients with atopic dermatitis. 885 37

Pain relief mechanisms of needling to the pain-producing muscle, application of a static magnetic field or external qigong, and needling to the acupuncture point were investigated in an experimentally designed pain producing muscle of animals. Single isometric twitch height in situ was reduced gradually by 10 Hz tetanic stimulation for one hour of the gastrocnemius muscle of guinea pigs. This reduction of twitch height was recovered by injection of 0.3-1 ml saline to the artery of this muscle, or of injection of a vasodilator, isoproterenol dissolved in 0.1 ml saline. Hence, reduction of twitch height could be induced by reduction of circulation in the muscle and recovery of it could be induced be recovery of circulation. Since it is easily considered that a pain substance might be accumulated in a muscle under reduced circulation, and such an accumulated substance might be eliminated by recovery of circulation, the reduction of twitch height after tetanic stimulation could be estimated as the pain-producing muscle and recovery of twitch, as the pain relieving muscle. 1) Needling to the pain muscle, 2) application of a static magnetic field or external qigong to the muscle, and 3) needling to the acupuncture point recovered the reduced twitch height due to tetanic stimulation. Atropine abolished this effect induced by the above 1, 2 and 3 procedures. Hence, the cholinergic vasodilator nerve might be involved in the induction of this effect. A sciatic nerve cut did not influence the effect of 1), but abolished the effect of 3). Denervation and capsaicin abolished the effect of 1). Substance P and a calcitonin gene- related peptide (CGRP) recovered the reduced twitch height, and atropine blocked the effect of CGRP, but did not block that of substance P. The effect of 2) was equivalent to that of anticholinesterase. A rostral lesion of the contralateral anterior hypothalamus did not abolish the effect of 3, but a caudal lesion of this region did. Electrical stimulation of this region produced an effect similar to that of 3). From these results, it was concluded that muscle pain relief by these procedures might be induced by recovery of circulation due to the enhanced release of acetylcholine as a result of activation of the cholinergic vasodilator nerve endings innervated to the muscle artery. However, manners of activation of the cholinergic nerve was different in effects of 1), 2) and 3). 1) might be induced by axon reflex of the CGRP nerve, 2) might be induced by inhibition of cholinesterase and 3) might be induced by a somato-autonomic reflex. The reflex center of this might be in the anterior hypothalamus.
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PMID:Comparisons of pain relief mechanisms between needling to the muscle, static magnetic field, external qigong and needling to the acupuncture point. 891 86

Administration of monoclonal antibodies against acetylcholinesterase (AChE-mabs) to adult rats leads to a selective degeneration of the acetylcholine esterase-(AChE), choline acetyltransferase-(ChAT) and enkephalin-(ENK) positive preganglionic fibres of the splanchnic nerve innervating the adrenal gland. Here we used this approach of immunological sympathectomy, performed at postnatal day 2 (P2), in an attempt to study the development role of the preganglionic fibres in the adrenal medulla in more detail. Analysis was performed at P16 and revealed that the effect of this treatment varied considerably between animals, as judged by the number of remaining AChE-, ChAT- and ENK-positive fibres. The number and intensity especially of ENK fibres in the adrenal medulla correlated negatively with the number and staining intensity of ENK-immunoreactive chromaffin cells, suggesting a 'dose-response' relationship. Thus, the high early postnatal levels of ENK-like immunoreactivity generally persisted in chromaffin cells of adrenals with a successful immunosympathectomy, i.e. in those adrenals that lacked AChE-, ChAT- and ENK-positive nerves. In contrast, calcitonin gene-related peptide-like immunoreactivity in nerves and chromaffin cells was not affected. Large and strongly AChE-positive intra-adrenal ganglion neurones, recently termed type I ganglion neurones, were present also after AChE-mab treatment and had an apparently normal morphology. These results indicate a role for preganglionic fibres in the developmental regulation of ENK in the chromaffin cells. However, these fibres appear less important for the postnatal development of the type I ganglion neurones.
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PMID:Effects of immunological sympathectomy on postnatal peptide expression in the rat adrenal medulla. 894 57

We studied the location, distribution, and density of uterine nerve bundles in virgin, full-term pregnant, and early postpartum female rats. In the virgin, a low-magnification analysis of uterine whole-mounts stained for acetylcholinesterase revealed fibers both in close association with blood vessels and coursing freely within muscle or connective tissue in the mesometrium and the uterine body. Higher magnification analysis of sections treated immunohistochemically with antibodies to the ubiquitous neuronal protein gene product 9.5, tyrosine hydroxylase, and calcitonin gene-related peptide indicated that the greatest density of nerve bundles was in the mesometrial triangle and intramyometrial region. Although the majority of bundles were associated with blood vessels, many coursed freely, particularly in the myometrial and endometrial layers. The density of innervation within the body of the uterus was heterogeneous. For instance, both innervated and noninnervated spiral arteries branched from the same innervated circumferential artery. In the term pregnant rat, we observed a profound denervation within all layers of the body of the uterus. The few remaining nerve bundles tended to be associated with blood vessels, and they were confined to the intramyometrial region. Within 48 h postpartum, both the number and density of nerve bundles increased. Long tortuous bundles, not seen in the full-term rat, were observed. The present study is the first to describe and illustrate the pattern of uterine innervation in virgin, full-term pregnant, and early postpartum rats over a wide range of magnifications.
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PMID:Pregnancy-induced uterine neuronal degeneration in the rat. 908 65


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