Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.1.7 (acetylcholinesterase)
 28,390 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We studied 5'-nucleotidase in preparations of synaptic plasma membranes from bovine caudate nucleus. The best substrates for this membrane-bound enzyme were purine nucleotides, particularly 5'AMP. Effects of metal cations and chelating agents suggest that 5'-nucleotidase is a metalloprotein. Optimal conditions for solubilization of the 5'-nucleotidase were found by using a low concentration of the zwitterionic detergent sulfobetaine 14. In contrast, another membrane-bound enzyme, acetylcholinesterase, was not solubilized under these conditions, but only in the presence of Triton X-100. The effects of lectins (concanavalin A, Lens culinaris agglutinin, wheat germ agglutinin, and Limulus polyphemus agglutinin) showed that both enzymes are glycoproteins. Sequential hydrolysis with specific glycosidases produced modifications of the effect of lectins on these enzymes. The results suggest the presence of a complex-type glycosylation, with a fucose residue on the internal N-acetyl-D-glucosamine of the pentasaccharide core.
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PMID:5'-Nucleotidase from bovine caudate nucleus synaptic plasma membranes: specificity for substrates and cations; study of the carbohydrate moiety by glycosidases. 632 59

In order to approach practicable indexes for assessing the effects of the products which are intended to be used to prevent endemic fluorosis, Wistar rats were fed with fluoridated water(50 mg/L) for four weeks. After only one week treated with fluoride, a higher activity of NAG(N-acetyl-beta-D-glucosaminidase) in urine, ChE(cholinesterase) in blood and increased urinary excretion of fluoride (compared with the control, P < 0.01) were observed, and these changes were lasted to the end of the fourth week of the study, but hydroxyproline in serum and urine had not been changed in both groups. After four weeks, the fluoride levels in the femora and teeth of fluoride treated group were ten times higher than that of the control group. According to the results, fluoride in bones and teeth must be taken as key indexes, the activities of NAG in urine and ChE in blood should be taken into consideration for assessing the effects of fluoride protagonist.
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PMID:[Effects of high fluoride intake on the fluoride of femora, teeth and some biochemical indexes in rats]. 1201 82