Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.1.7 (
acetylcholinesterase
)
28,390
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The objective of this study was to investigate the potential effect of the polysaccharides isolated from
Hericium novae-zealandiae
, a native New Zealand fungus, on the
in vitro
proliferation of prostate cancer cell lines, gene expression,
acetylcholinesterase
(
AChE
) activity, and oxidation. One water-soluble and two alkali-soluble polysaccharide fractions were isolated from
H. novae-zealandiae
. The proliferation of the prostate cancer cell lines DU145, LNCaP, and PC3 was evaluated following treatment with these polysaccharide fractions. It was found that the polysaccharides possess anti-proliferative activity on LNCaP and PC3 cells, with a 50% growth inhibition (IC
50
) value as low as 0.61 mg/mL in LNCaP. Subsequently, it was determined through via RT-qPCR assay that apoptosis was one of the possible mechanisms responsible for the anti-proliferative activity in LNCaP. This was supported by the up-regulation of
CASP3
,
CASP8
, and
CASP9
. An alternative, discovered in PC3, was revealed to be anti-inflammation, which was hinted at by the down-regulation of
IL6
and up-regulation of
IL24
. The polysaccharides also exhibited antioxidant and weak
AChE
inhibitory activities. This is the first report on the potential health benefits of polysaccharides prepared from the New Zealand fungus,
H. novae-zealandiae
.
...
PMID:Assessment of In Vitro Bioactivities of Polysaccharides Isolated from
Hericium
Novae-Zealandiae
. 3128
