Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:3.1.1.7 (acetylcholinesterase)
28,390 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The distribution of choline acetyltransferase (ChAT)-containing neurons and serotonin (5-HT)-containing nerve fibers in the cat neostriatum was investigated by use of immunohistochemical techniques. Both ChAT- and 5-HT-staining techniques were applied to alternate brain sections, thereby allowing a precise comparison of the distribution pattern of ChAT-immunopositive cells (ChAT cells) and 5-HT-immunopositive fibers (5-HT fibers). In the neostriatum, ChAT cells were strongly stained throughout their cell bodies and proximal (first-order) dendrites. The majority of them were multipolar cells with a soma diameter of 20-50 microm (long axis)x10-30 microm (short axis). In the caudate nucleus, ChAT cells were evenly and diffusely distributed except for the dorsolateral region of its rostral half, in which latter region they were distributed in loosely formed clusters. In the rostral portion of the putamen, the density of ChAT-cell distribution was like that in the medial region of the caudate nucleus. In contrast, this distribution was more dense in the caudomedial region of the putamen, adjacent to the globus pallidus. 5-HT fibers in the neostriatum were dark-stained, of quite fine diameter (<0.6 microm), and they contained small, round varicosities (diameter, usually 0.5-1.0 microm, but some >1.0 microm). Such 5-HT fibers were distributed abundantly throughout the caudate nucleus and putamen. In the rostrocaudal portion of the caudate nucleus, their density was high in its dorsal and ventral components, and low in the middle component. Throughout the putamen, 5-HT fibers were distributed homogeneously in the mediolateral and dorsoventral directions. In the caudal portion of the putamen adjacent to the globus pallidus, the 5-HT fibers had a higher density while maintaining their homogenous distribution pattern. In the two main divisions of the striatum, the so-called 'patch' (acetylcholinesterase (AChE)-poor) and 'matrix' (AChE-rich) compartments, there was a near-even distribution of 5-HT fibers and terminals. The above results suggest that the 5-HT-dominated, raphe-striatal pathway is optimally arranged for modulating the activity of both the intrinsic and the projection neurons of the neostriatum.
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PMID:The cat neostriatum: relative distribution of cholinergic neurons versus serotonergic fibers. 1098 58

The aim of this study was the description of the morphology and distribution of nerve structure elements in the intestine of the lizard Podarcis hispanica using different histochemical methods; namely acetylcholinesterase (AChE), formol-induced fluorescence for catecholamines (FIF), nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), and immunohistochemistry for vasoactive intestinal peptide (VIP), as well as substance P (SP) and electron microscopy. The AChE method showed fibres in the myenteric and submucosal plexus, with a higher fibre density in the large intestine. The highest number of related neurons was located in the myenteric plexus ganglia. Noradrenergic innervation was distributed through the myenteric and submucosal plexus, and also around blood vessels, with the highest fibre density in the large intestine. VIP immunohistochemistry showed a wide distribution of positive fibres throughout the intestine, although the highest density was again detected in the large intestine. Small positive cells for VIP were located at internodal segments in the plexus. SP labeling, although subtle, was present all along the intestine. It showed delicate varicose nets and few fibres innervating blood vessels. Small positive cells for SP were located in the large intestine. The indirect method to detect nitric oxide (NO)-producing system showed neural cells in the myenteric plexus ganglia of the large intestine. Electron microscopy showed ganglion neurons with scattered chromatin condensations, glial cells with higher electron density, and axons with varicosities occupied by different vesicles. We also identified certain cells as interstitial cells of Cajal due to their ultrastructural features. They were mostly located in the region of the myenteric plexus.
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PMID:Intrinsic innervation in the intestine of the lizard Podarcis hispanica. 1100 34

Studies of W/W(V) mice, which lack intramuscular interstitial cells of Cajal (IC-IM), have suggested that IC-IM act as mediators of enteric motor neurotransmission in the gastrointestinal tract. We have studied Sl/Sl(d) mice, which lack the ability to make membrane-bound stem cell factor, to determine the consequences of inappropriate stem cell factor expression on IC-IM populations and on enteric motor neurotransmission. IC-IM were found within the circular and longitudinal muscles of the gastric fundus of wild-type mice. IC-IM were intimately associated with motor nerve terminals and nerve varicosities formed synaptic structures with these cells. IC-IM were also connected with neighbouring smooth muscle cells via gap junctions. Immunohistochemistry and electron microscopy showed that IC-IM were absent from fundus muscles of Sl/Sl(d) mice, but the density of excitatory and inhibitory nerves was not significantly different than in wild-type muscles. Loss of IC-IM was associated with decreased membrane noise (unitary potentials) and significant reductions in post-junctional excitatory and inhibitory enteric nerve responses. Reductions in neural responses were not due to defects in smooth muscle cells as responses to exogenous ACh and K(+)-induced depolarization were normal in Sl/Sl(d) mice. Responses to neurally released ACh were revealed in Sl/Sl(d) mice by inhibiting ACh breakdown with the acetylcholinesterase inhibitor neostigmine. Inhibitory nerve stimulation elicited inhibitory junction potentials (IJPs) and relaxations in wild-type mice. IJPs were reduced in amplitude and relaxation responses were absent in Sl/Sl(d) mice. These observations suggest that membrane-bound stem cell factor is essential for development of IC-IM and that the close, synaptic-like relationship between nerve terminals and IC-IM may be the primary site of innervation by enteric motor neurons in gastric muscles.
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PMID:Loss of enteric motor neurotransmission in the gastric fundus of Sl/Sl(d) mice. 1223 45

This study was designed to elucidate whether the fibrosis index (FI), which was measured as a ratio of histological fibrotic tissue area to the whole area using an image analyzer, could reflect liver function and long-term prognosis in biliary atresia (BA). Liver biopsies were performed in 46 BA patients at hepatic portoenterostomy (HPE) and stoma closure. The chronological difference rate of FI (FIDR) was the monthly FI difference between HPE and stoma closure. FI at HPE and stoma closure was significantly higher than in the control. FI at HPE and at stoma closure significantly correlated with gammaGTP or T.Bil, D.Bil, cholinesterase and total bile acid, respectively. FIDR in jaundice-free group was significantly lower than in jaundiced group at 5 years after HPE. FIDR in V-2 (varices with red-color sign) was significantly higher than in V-0 (no varices) or V-1 (varices without red-color sign). ICG-K value significantly correlated with FIDR. FI at stoma closure or FIDR was significantly lower in living patients than in patients who eventually died or underwent liver transplantation. In conclusion, FI can reflect the degree of cholestasis in BA. FIDR would be useful for predicting long-term outcome in BA.
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PMID:Proposal of fibrosis index using image analyzer as a quantitative histological evaluation of liver fibrosis in biliary atresia. 1272 24

In vivo, the abundance of receptors at the neuronal plasma membrane may be critical in the mediation of pre- and postsynaptic responses. Thus, we have studied the membrane availability and intraneuronal distribution of the m2 muscarinic autoreceptor (m2R) in cholinergic neurons of the nucleus basalis magnocellularis (NBM) projecting to the frontal cortex (FC). We have studied the subcellular compartmentalization of m2R at somatodendritic postsynaptic and axonal presynaptic sites in control animals (AChE +/+) and in two animal models: mice displaying acute acetylcholinesterase (AChE) inhibition by treatment with metrifonate, and AChE-deficient mice (AChE -/-). In control animals, m2R was mainly located at the plasma membrane in the somatodendritic field of NBM and in cortical varicosities. Acute AChE inhibition and chronic AChE deficiency induced a dramatic decrease of cell surface m2R in the somatodendritic compartment. This finding was associated with two different intracytoplasmic events: (1). internalization of m2R in endosomes after acute AChE inhibition, (2). exaggerated storage of m2R in the endoplasmic reticulum and Golgi complex in AChE -/- mice. In contrast, the m2R density was higher at the membrane of cortical varicosities in AChE -/- mice but unchanged in acutely AChE-inhibited mice. Our data demonstrate that acute and chronic stimulation provoke, in vivo, depletion of the membrane store of somatodendritic m2R through different intracellular mechanisms: endocytosis of receptors from the plasma membrane to the cytoplasm (acute) or regulation of their delivery from intracytoplasmic stores to the plasma membrane (chronic). The increase of m2R at the membrane of axonal varicosities after chronic stimulation suggest modulation of presynaptic cholinergic activity, including neurotransmitter release.
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PMID:Trafficking of the muscarinic m2 autoreceptor in cholinergic basalocortical neurons in vivo: differential regulation of plasma membrane receptor availability and intraneuronal localization in acetylcholinesterase-deficient and -inhibited mice. 1279 34

The neurotoxic effects of amyloid-beta(1-42) and amyloid-beta(25-35) (A beta) on cholinergic and acetylcholinesterase-positive neurons were investigated in primary cultures derived from embryonic 18-day-old rat basal forebrain. After various time intervals, the cultures were treated with 1, 5, 10 or 20 microM A beta for different time periods. The cholinergic neurons and their axon terminals were revealed by vesicular acetylcholine transporter immunohistochemistry and the cholinoceptive cells by acetylcholinesterase histochemical staining. To assess the toxic effects of these A beta peptides on the cholinergic neurons, image analysis was applied for quantitative determination of the numbers of axon varicosities/terminals and cells. The results demonstrate that, following treatment with 1 or 5 microM A beta for 5, 10, 30, 60 or 120 min, no changes in vesicular acetylcholine transporter immunohistochemical staining were observed. However, after treatment for 30 min with 10 or 20 microM A beta, the number of stained axon varicosities was reduced, and treatment for 2 h they had disappeared. In contrast, vesicular acetylcholine transporter-positivity could be seen in some of the neuronal perikarya even after 3 days after treatment. The acetylcholinesterase staining was homogeneously distributed in the control neurons. After A beta treatment, the histochemical reaction end-product was detected in some of the neuronal perikarya or in the dendritic processes near to the soma. It is concluded that the neurotoxic effects of A beta appear more rapidly in the cholinergic axon terminals than in the cholinergic and acetylcholinesterase-positive neuronal perikarya.
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PMID:Effects of amyloid-beta on cholinergic and acetylcholinesterase-positive cells in cultured basal forebrain neurons of embryonic rat brain. 1472 70

Striatal projections from the lateral intermediate (LI) and posterior (Po) thalamic complexes were studied with the anterograde tracers wheat germ agglutinin-horseradish peroxidase and Phaseolus vulgaris leucoagglutinin. Projections to the lateral part of the head and body of the caudate nucleus (CN) and to the putamen (Pu) were found to arise from the ventral parts of the caudal subdivision of the LI besides the well established sources in the intralaminar and ventral thalamic nuclei. No projections to the CN and only a few to the Pu were found to arise from the medial division of the Po. The presence of terminal and intercalated varicosities in the thalamostriatal fibers suggests that they form both terminal and en passant synapses. Thalamostriatal fibers from these thalamic sectors were unevenly distributed within the CN, with patches of either low-density innervation or with no projections at all interspersed within irregular, more densely innervated areas. The former coincided with the acetylcholinesterase-poor striosomes and the latter areas of dense projection with the extrastriosomal matrix.
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PMID:Striatal projections from the lateral and posterior thalamic complexes. An anterograde tracer study in the cat. 1619 93

Many teleosts actively regulate buoyancy by using a gas-filled swim bladder, which is thought to be under autonomic control. Here we investigated the swim bladder in the zebrafish to determine possible mechanisms of gas-content regulation. Fluorescently labelled phalloidin revealed myocytes that appeared to form a possible sphincter at the junction of the pneumatic duct and esophagus. Myocytes also formed thick bands along the ventral surface of the anterior chamber and bilaterally along the posterior chamber. Thinner layers of myocytes were located elsewhere. Staining of peroxidase within erythrocytes revealed a putative rete and smaller blood vessels in muscle bands and elsewhere. The antibodies zn-12, a general neuronal marker, and SV2, a synaptic vesicle marker labelling presynaptic terminals, revealed widespread innervation of the swim bladder system. Widespread innervation of the swim bladder was also indicated by acetylcholinesterase histochemistry, but choline acetyltransferase-immunoreactive (-IR) somata and fibers were limited to the junction of the pneumatic duct and esophagus. In contrast, varicose tyrosine hydroxylase-IR fibers innervated muscles and blood vessels throughout the system. Neuropeptide Y-IR somata were located near the junction of the duct and esophagus and varicose fibers innervated muscles and vasculature of the posterior chamber and duct. Vasoactive intestinal polypeptide immunoreactivity was abundant throughout the anterior chamber but sparsely distributed elsewhere. Serotonin-IR fibers and varicosities were located only along blood vessels near the junction of the pneumatic duct and posterior chamber. Our results suggest that the zebrafish swim bladder is a complex and richly innervated organ and that buoyancy-regulating effectors may be controlled by multiple populations of autonomic neurons.
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PMID:Structure and autonomic innervation of the swim bladder in the zebrafish (Danio rerio). 1649 79

A 25-35% reduction of brain cytochrome oxidase (COx) activity found in Alzheimer's disease (AD) could contribute to neuronal dysfunction and cognitive impairment. The present study replicated the reduction in brain COx activity in rats by administering sodium azide (NaN(3)) for 4 weeks via Alzet minipumps at the rate of 1 mg/kg/h, and determined its effect on hippocampal cholinergic transmission, spatial and episodic memory. NaN(3) caused a selective reduction in choline acetyltransferase (ChAT) immunoreactivity in the diagonal band, a major source of cholinergic input to the hippocampus and cingulate cortex, without altering the number of cholinergic neurons. NaN(3) also induced a significant increase in vesicular acetylcholine transporter (VAChT)-immunoreactive varicosities, GAP-43 in the subgranular layer and of transferrin receptors (TfR) in the hilus of the dentate gyrus. These neurochemical changes were associated with impairment in spatial learning in the Morris water maze and in episodic memory in the object recognition test. Chronic treatment with ladostigil, a novel cholinesterase and monoamine oxidase inhibitor, prevented the decrease in ChAT in the diagonal band, the compensatory increase in synaptic plasticity and TfR and the memory deficits without restoring COx activity. Ladostigil had no significant effect on ChAT activity, synaptic plasticity or TfR in control rats. Ladostigil may have a beneficial effect on cognitive deficits in AD patients that have a reduction in cortical COx activity and cholinergic hypofunction.
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PMID:Chronic brain cytochrome oxidase inhibition selectively alters hippocampal cholinergic innervation and impairs memory: prevention by ladostigil. 1758 85

The usefulness of endoscopic ultrasonography (EUS) in the evaluation of rectal varices (RV) was determined in 50 patients with portal hypertension (PH) and 25 PH-free controls. F(1) and F(2) varices and angiectasia were specific for the PH group as evaluated by endoscopy, but there was no difference between the PH and the control groups with respect to the frequency of blue vein. The detection rate of submucosal veins (SMV) with EUS was 88% for the PH group and 68% for the control group. The mean SMV diameter was significantly greater for the PH group than for the control group, and no 2-mm or larger SMV was detected in the control group. Serum albumin and cholinesterase levels were significantly higher for the RV(+) patients with SMV 2mm or more in diameter in the PH group than for the RV(-) patients. The spleen index was also significantly higher for the former group. The frequency of RV was significantly higher for advanced PH than for mild PH. RV(+) was detected in about 30% of endoscopically normal patients in the PH group. The results of this study indicate that EUS is useful in detecting RV and evaluating its pathological condition.
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PMID:Evaluation of rectal varices by endoscopic ultrasonography in patients with portal hypertension. 1849 61


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