Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.1.53 (sialidase)
 2,694 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Human anti-S and anti-s eluates bound to glycophorin B on immunoblots from membranes of S+ and s+ red cells, respectively. Eluates of human anti-S were more efficiently prepared from sensitized trypsin-treated cells than from sensitized untreated cells. The results of immunoblotting membranes from enzyme-treated cells confirmed the serological findings: S and s antigens were not affected by treatment with trypsin or sialidase but were destroyed or much depressed by treatment with papain, pronase or alpha-chymotrypsin. Immunoblotting with anti-S or anti-s of membranes from cells with unusual MNS phenotypes confirms the involvement of glycophorin B in hybrid glycophorins; the existence of such hybrid glycophorins was deduced previously from serological work or immunoblotting with monoclonal antibodies. The presence of s-active glycophorin B in glycophorin (B-A)Dantu, in glycophorin BMiIII and in glycophorin (A-B)MiV was confirmed. The bands observed when Mit+ membranes were immunoblotted with anti-S supports the suggestion from serological work that the Mit antigen is associated with an altered S antigen.
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PMID:Immunoblotting of human red cell membranes: detection of glycophorin B with anti-S and anti-s antibodies. 239 72

Whereas monoclonal reagents for ABO, Rhesus, Kell, Kidd, Lewis and MNS antigens are widely used in blood grouping laboratories and have been well evaluated, little attention has been given so far to monoclonal anti-P1 reagents. Therefore it was the aim of our study to examine the suitability of monoclonal anti-P1 reagents for routine use in comparison to polyclonal anti-P1 reagents. 10 polyclonal anti-P1 reagents (9 from goat, 1 from rabbit) and 5 monoclonal anti-P1 reagents (4 with clone 650, 1 with clone OSK17) were tested by the tube-spin-method at room temperature (incubation time as prescribed by the manufacturer) using untreated P1+ red blood cells (RBCs) for titration and untreated P1- RBCs as negative controls. P1- RBCs with a positive direct anti-globulin test (DAT+) (coated with incomplete anti-D) and sialidase-treated P1- RBCs (T+) were used to recognize false positive reactions. 3 monoclonal anti-P1 reagents had a titer of < or = 1 with P1+(weak) RBCs, but all polyclonal anti-P1 reagents showed a titer > or = 2. Negative controls (untreated P1- RBCs) were inconspicuous. 7 polyclonal and 1 monoclonal anti-P1 reagents showed false positive reactions with P1- DAT+ RBCs. All polyclonal and 1 monoclonal (containing clone OSK17) reagent showed false positive reactions with P1- T+ RBCs. As several monoclonal anti-P1 reagents are less suitable for routine use than polyclonal anti-P1 reagents, quality control of anti-P1 reagents should be intensified.
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PMID:Comparison of monoclonal and polyclonal anti-P1 reagents. 1140 3