Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.1.53 (sialidase)
 2,694 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A multi-coupled enzyme assay system for determining sialidase activity is described. Enzymes, substrates and chromogens are reacted in situ and determined spectrophotometrically in ELISA microtiter plates. Sialidase is assayed by the extent of desialylated galactose on an appropriate sialoglycoconjugate (fetuin), which is otherwise unavailable for oxidation by galactose oxidase. The oxidation is monitored by the coupling of H2O2 released to a third enzyme, peroxidase. The rate of change of absorbance at 405 nm, resulting from the oxidized chromogen is a measure of the reaction rate of the coupled enzyme system. A similar system can be used for determining galactose oxidase in solution, or on blots using galactose as substrate. Due to the small-scale single-step measurement, the described assay is a sensitive, convenient, and inexpensive alternative to the classic colorimetric determination.
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PMID:A coupled enzyme assay for measurement of sialidase activity. 170 71

The vaginal flora of 118 Japanese and 208 Thai pregnant women were investigated for the presence of bacterial vaginosis (BV), BV-associated organisms, and BV-associated enzyme. A similar prevalence of BV was found among the Japanese (13.6%) and Thai women (15.9%). The microbial flora of women with BV were complex; the mean number of isolates recovered in the BV group was approximately 2 times more than that in a group of healthy women. Prevotella species, Porphyromonas species, Peptostreptococcus species, Mobiluncus species, Gardnerella vaginalis, and H2O2-nonproducing lactobacilli were significantly associated with BV. These organisms were less associated with H2O2-producing lactobacilli, which were predominant in women with normal flora, suggesting that H2O2-producing lactobacilli have antibacterial activity against BV-associated organisms. The vaginal sialidase assay by means of a filter-paper spot test was not proved to be a useful screening aid for diagnosis of BV because of the low sensitivity (69.4%) of this test.
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PMID:Vaginal microflora associated with bacterial vaginosis in Japanese and Thai pregnant women. 890 38