Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.1.53 (
sialidase
)
2,694
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In our previous study, a Burkitt lymphoma-associated antigen defined by a monoclonal antibody, designated 38.13, was characterized as globotriaosylceramide (
Gb3
, Gal alpha 1----4 Gal beta 1----4 Glc beta 1----1 Cer) (Nudelman, E., Kannagi, R., Hakomori, S., Parsons, M., Lipinski, M., Wiels, J., Fellous, M., and Tursz, T. (1983) Science (Wash. D.C.) 220, 509-511). Consequently, we have studied the enzymatic basis and organization of
Gb3
expression in Burkitt as compared with non-Burkitt lymphoblastoid cell lines. Burkitt lymphoma cell lines (Ramos, Daudi, Put) were characterized by a high chemical quantity of
Gb3
, high enzyme activity for synthesis of
Gb3
(UDP-Gal:LacCer alpha-galactosyltransferase), and a high degree of surface exposure of
Gb3
, as determined by galactose oxidase/NaB[3H]4 and by cytofluorometry with the monoclonal antibody to
Gb3
(38.13). Non-Burkitt lymphoblastoid cell lines (Priess, Remb1, and ARH77) were characterized by the absence of
Gb3
at the cell surface detected by cytofluorometry or cell-surface labeling. The cell lines Priess and Remb1 did not contain
Gb3
and showed a low alpha-galactosyltransferase activity for
Gb3
synthesis. However, the cell line ARH77, though it did not express
Gb3
at the cell surface, was found to contain a large chemical quantity of
Gb3
and a high level of alpha-galactosyltransferase activity for
Gb3
synthesis. However,
Gb3
of ARH77 cells was exposed by
sialidase
treatment, but not by protease treatment, although
Gb3
itself was not sialylated. The crypticity of
Gb3
in ARH77 cells could be associated with an adjacent sialosyl residue of a second glycoconjugate at the cell surface, in the same way as Gg3 in mouse lymphoma L5178 (Urdal, D. L., and Hakomori, S. (1983) J. Biol. Chem. 258, 6869-6874). Thus, the expression in Burkitt and non-Burkitt lymphoma is dependent on (i)
Gb3
synthesis due to alpha-galactosyltransferase activity and (ii) membrane organization of
Gb3
, which may be controlled through interaction with the sialosyl residue of a second glycoconjugate.
...
PMID:Enzymatic and organizational difference in expression of a Burkitt lymphoma-associated antigen (globotriaosylceramide) in Burkitt lymphoma and lymphoblastoid cell lines. 643 3
