Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.1.53 (sialidase)
 2,694 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Physicochemical characteristics of an anti-LH substance, partially purified from human urine, has been determined. It is an acid-precipitable, sialic acid containing glycoprotein with a molecular weight of 162,000. This substance on mg to mg basis has a 50 fold biological potency when compared to that of the crude gonadotropin inhibiting material. Dialysed human pineal protein extracts also showed anti-LH activity which was not attributed to sialidase activity. Polyacrylamide gel electrophoresis of such pineal extracts revealed a glycoprotein component with the same mobility as that of the urinary anti-LH protein. These observations suggest a possibility that the human pineal gland may be associated either with secretion or storage of this gonadotropin inhibiting entity.
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PMID:Some observations on gonadotropin inhibitor (anti-LH) for human urine--with discussion on its possible source of origin. 91 52

Polyacrylamide gels were stained with the sialidase substrate 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid showing the activity of Vibrio cholerae and Clostridium sordellii sialidases in the gels after electrophoresis. With this fluorogenic method minimum sialidase activities of 5 microU could be determined. The sensitivity of this staining is about 10,000-fold higher compared to protein-staining with Coomassie brilliant blue. For the visualization of other proteins than sialidases the specific sialidase staining could be followed by a protein-staining method in the same gel.
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PMID:Fluorescent staining of sialidases in polyacrylamide gel electrophoresis and ultrathin-layer isoelectric focusing. 240 36