Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.1.5 (neuropathy target esterase)
 1,070 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Acidic and basic lysophospholipase activities (LPL) have been separated by ion-exchange chromatography of barley extracts. The basic activity predominates in the starchy endosperm of germinating barley and in the medium of hormone-stimulated half-seeds; the acidic activity is the predominant form in the medium of hormone-stimulated aleurone layers. Addition of either starchy endosperm or EDTA to the acidic activity produces the basic activity. The two activities display the same pH optimum and have similar Km values. Inactivation profiles of LPLs with immunoglobulin G (IgG) prepared against the purified basic LPL are the same. The acidic LPL obtained from the incubation medium from stimulated aleurone layers appears in the void volume on gel filtration with Bio-Gel P100. Acid phosphatase and alpha-amylase in the same incubation medium appear at their expected elution volumes on this column. Gel filtration in the presence of EDTA results in the acidic activity eluting in a volume characteristic of the basic LPL (Mr, 40,000). On Bio-Gel P300 the acidic activity peak is centered at Mr, 160,000. SDS-gel electrophoresis of fractions across this peak shows a simple distribution of proteins eluting with Mr greater than or equal to 160,000. The potential role of an aggregate in the secretion of lipolytic proteins is discussed.
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PMID:Secretion of a lipolytic protein aggregate by barley aleurone and its dissociation by starchy endosperm. 375 11

A lysophospholipase (LPL) activity appears in the aleurone of barley (Hordeum vulgare L. cv Himalaya) half seeds during imbibition on moist agar. Secretion of LPL by half seeds is promoted by GA(3); the increase in secretory rate is almost linear from 10(-10) to 10(-6) molar GA(3). LPL activity is likewise promoted in isolated aleurone layers by GA(3). Its secretion into the incubation medium requires the continued presence of GA(3) and commences after a 10 to 14 hour lag period when 10 millimolar Ca(2+) is present. In the absence of Ca(2+), the lag period remains unchanged but attainment of the maximum secretory rate is delayed. Ca(2+) alone has very little effect either on LPL activity accumulated in the aleurone layer or in the surrounding medium. However, 50 millimolar Ca(2+) together with GA(3) dramatically increase the level of secreted activity and of total (accumulated and secreted) activity.The metabolic inhibitors cycloheximide and actinomycin D inhibit the accumulation of LPL activity in the aleurone and also the secreted activity. Actinomycin D added after the lag period results in a much lower inhibition. The increase in LPL activity in response to GA(3) occurs as a result of de novo synthesis; LPL activity from barley half seeds incubated in 80% D(2)O in the presence of GA(3) undergoes a shift to higher density compared with the activity from similar controls incubated in H(2)O. The characteristics of the GA(3) enhancement of LPL activity are compared specifically with alpha-amylase and generally with other GA(3)-controlled hydrolases.
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PMID:Characterization of the increased lysophospholipase activity in gibberellic Acid-treated barley aleurone layers. 1666 37