Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.1.34 (lipoprotein lipase)
 7,025 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The fractional elimination rate of fat emulsions of soybean oil, emulsified with egg yolk phosphatides with 1% addition of cholesterol or various cholesteryl fatty acid esters, was studied in rabbits. The fractional removal rate k2%/min was the same after addition of free cholesterol or esters with fatty acids up to eight carbon esters. The k2 values were twice as high for emulsions with cholesteryl-stearate, three times higher with added cholesteryl-palmitate and four times higher when cholesteryl-linoleate was added. The triglyceride (TG) lipase activity was determined with human or rabbit post-heparin plasma and with purified bovine lipoprotein lipase. All these enzyme sources gave similar results. Addition of saturated cholesteryl esters did not affect the lipase activity, but addition of 1% cholesterol markedly decreased the lipase activity. Furthermore, addition of cholesteryl-linoleate and linolenate reduced post-heparin TG lipase activity.
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PMID:Fat emulsions with added free cholesterol or fatty acid cholesteryl esters. Studies on removal mechanisms in vivo and hydrolysis by lipoprotein lipase in vitro. 56 51

The peroxisome proliferator-activated receptors (PPAR) are critical for lipid metabolism, and many fatty acids are PPAR agonists. Madin-Darby bovine kidney (MDBK) cells were tested as an in vitro bovine model for PPAR activation, and preliminary evaluation of the effect of fatty acids on bovine PPAR was performed. Cells were treated with Wy-14643 (WY, specific PPARalpha agonist) and rosiglitazone (ROSI, specific PPARgamma agonist). The gene expression of specific PPARalpha-responsive genes such as carnitine palmitoyl transferase-1 (CPT1A) and acetyl coenzyme A oxidase (ACOX1) and of PPARgamma-responsive gene lipoprotein lipase (LPL) were analyzed using real-time reverse transcription PCR. It was found that CPT1A exhibited a significant increase in cells treated with WY, whereas the ACOX1 gene expression was not altered. The LPL gene expression showed an increase in response to ROSI. Interestingly, LPL was almost undetectable in MDBK cells not treated with ROSI. The potency of different fatty acids in activating PPARalpha as assessed by CPT1A mRNA abundance in MDBK cells was also tested. The mRNA of CPT1A (2.5- to 1.4-fold) was significantly increased by fatty acids in the order of palmitate > linolenate > linoleate > conjugated linoleate, and oleate. The results demonstrated MDBK cells to be responsive to PPAR agonists and thus a promising model to evaluate the role of PPAR in bovine cells. In addition, fatty acids were proven to have a different potency in modulating expression of CPT1A through PPARalpha.
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PMID:Characterization of Madin-Darby bovine kidney cell line for peroxisome proliferator-activated receptors: temporal response and sensitivity to fatty acids. 1856 38