Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.8 (
polynucleotide phosphorylase
)
723
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The PstI and BamHI fragments, containing the
HIS3
(imidazoleglycerol phosphate dehydratase) gene of yeast obtained from pYehis2, and the ColE1-derived plasmid pBR322 were ligated in vitro and used to transform hisB463 strains of Escherichia coli K-12. Expression of the cloned
HIS3
gene from yeast was markedly enhanced (3--5-fold) in
polynucleotide phosphorylase
(pnp)-deficient strains of E. coli. The levels of both
HIS3
and plasmid-encoded mRNAs were increased in pnp- strains carrying the chimeric plasmids, whereas there was little difference in the levels of pBR322-specific mRNAs in pnp+ and pnp- strains. This increase in
HIS3
mRNA appeared to be related to specific stabilization of the eukaryotic message due to its unique structural features, since the half-life of the
HIS3
mRNA increased from 1.5 to 18.7 min, whereas no increase in the half-lives of pBR322 vehicle mRNAs was observed. A physical map of the plasmid pYehis2 was constructed using restriction endonuclease and molecular cloning techniques.
...
PMID:Expression of the HIS3 gene of Saccharomyces cerevisiae in polynucleotide phosphorylase-deficient strains of Escherichia coli K-12. 701 1
