Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.7.7 (DNA polymerase)
 17,007 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The nucleotide sequence of maranhar, a senescence-inducing linear mitochondrial plasmid of Neurospora crassa, was determined. The termini of the 7-kb plasmid are 349-bp inverted repeats (TIRs). Each DNA strand contains a long open reading frame (ORF) which begins within the TIR and extends toward the centre of the plasmid. ORF-1 codes for a single-subunit RNA polymerase that is not closely related to that encoded by another Neurospora plasmid, kalilo. The ORF-2 product may be a B-type DNA polymerase resembling those encoded by terminal protein-linked linear genetic elements, including linear mitochondrial plasmids and linear bacteriophages. A separate coding sequence for the terminal protein could not be identified; however, the DNA polymerase of maranhar has an amino-terminal extension with features that are also present in the terminal proteins of linear bacteriophages. The N-terminal extensions of the DNA polymerases of other linear mitochondrial plasmids contain similar features, suggesting that the terminal proteins of linear plasmids may be comprised, at least in part, of these cryptic domains. The terminal protein-DNA bond of maranhar is resistant to mild alkaline hydrolysis, indicating that it might involve a tyrosine or a lysine residue. Although maranhar and the senescence-inducing kalilo plasmid of N. intermedia are structurally similar, and integrate into mitochondrial DNA by a mechanism thus far unique to these two plasmids, they are not closely related to each other and they do not have any nucleotide sequence features, or ORFs, that distinguish them clearly from mitochondrial plasmids which are not associated with senescence and most of which are apparently non-integrative.
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PMID:Genetic organization and structural features of maranhar, a senescence-inducing linear mitochondrial plasmid of Neurospora crassa. 142 26

The DNA polymerase (dnapol) gene of Autographa californica nuclear polyhedrosis virus presents a complex promoter organization. It lacks the usual TATA box and start site, and its RNA accumulation initially increases and then decreases dramatically during infection. We investigated dnapol temporal regulation. Transiently expressed dnapol gene was transcribed at a low level from minor start sites. Coexpression with ie0 and/or ie1 immediate-early genes dramatically enhanced dnapol transcription, specifically from a new start site. Moreover, the ie1 transactivation required little or no information in front of this nonconventional proximal promoter. We showed that IE0 and IE1 proteins were stably expressed during infection and that the dnapol mRNA level decrease was not a consequence of the disappearance of these proteins. The dnapol promoter region contains a putative overlapping open reading frame (ORF) in the opposite direction. We showed that ORF-2 was indeed highly expressed late, when the dnapol mRNA level decreased, and that during that time, dnapol mRNA stability was not significantly altered, excluding a destabilizing antisense effect. Additionally, we showed that the dnapol promoter was inhibited late but not early during the infection of cells transiently expressing constructs carrying either the intact or the altered ORF-2 promoter. Therefore, ORF-2 initiation of transcription and dnapol promoter inhibition are two coincidental nonrelated phenomena. Finally, we showed that both IE1 transactivation and late inhibition occurred in the same limited region around the dnapol promoter.
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PMID:Temporal regulation of a complex and unconventional promoter by viral products. 813 38