Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.7 (
DNA polymerase
)
17,007
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Objective To obtain the
PP7
bacteriophage-like particles carrying the peptide of prostatic acid phosphatase PAP
114-128
, and prove that they retain the original biological activity. Methods First, the plasmid pETDuet-2PP7 was constructed as follows: the gene of
PP7
coat protein dimer was amplified by gene mutation combined with overlapping PCR technology, and inserted into the vector pETDuet-1. Following that, the plasmid pETDuet-2PP7-PAP
114-128
was constructed as follows: the
PP7
coat protein gene carrying the coding gene of PAP
114-128
peptide was amplified using PCR, and then inserted into the vector pETDuet-2PP7. Both pETDuet-2PP7 and pETDuet-2PP7-PAP
114-128
were transformed into E.coli and expressed. The expression product was verified by SDS-PAGE, double immunodiffusion assay and ELISA. Results The gene fragment of
PP7
coat protein dimer was obtained by overlapping PCR using Ex
Taq DNA polymerase
, and the antigenicity of its expression product was the same as that of the coat protein of wild-type
PP7
bacteriophage. Moreover, the PAP
114-128
peptide epitope that was displayed on the surface of
PP7
bacteriophage was identical with the corresponding epitope of natural human PAP, and it was able to induce high levels of antibodies. Conclusion The gene of
PP7
coat protein dimer with repeated sequences can be prepared by gene mutation combined with overlapping PCR. Based on this,
PP7
bacteriophage-like particles carrying PAP peptide can be prepared, which not only solves the problem of the instability of the peptides, but also lays a foundation for the study on their delivery and function.
...
PMID:[Preparation and activity validation of PP7 bacteriophage-like particles displaying PAP
114-128
peptide]. 2766 62
