Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.7 (
DNA polymerase
)
17,007
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The majority of the
DNA polymerase alpha
activity in HeLa cells has been isolated and purified as a multiprotein Mr 640,000 form. The multiprotein form of
DNA polymerase alpha
corresponds to
DNA polymerase alpha
2 that was previously reported by us (Lamothe, P., Baril, B., Chi, A., Lee, L., and Baril, E. (1981) Proc. Natl. Acad. Sci. U. S. A. 78, 4723-4727). The highly purified
DNA polymerase alpha
2 has in addition to
DNA polymerase alpha
-associated DNase, primase, and diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A)binding activities and accessory primer recognition proteins C1 and C2. The
DNA polymerase alpha
and associated activities increase coordinately during the G1/S-phase transition of the cell cycle. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the electrophoretically homogeneous
DNA polymerase alpha
shows that it is composed of at least eight polypeptides in the molecular weight range of 180,000-15,000. Hydrophobic chromatography on butyl-agarose resolves the DNase and Ap4A-binding protein from a complex of
DNA polymerase alpha
, primase, and the primer recognition proteins C1 and C2. Hydrophobic chromatography of the latter complex on phenyl-Sepharose resolves the C1 protein from a
DNA polymerase alpha
-
C2 protein
-primase complex. Phosphocellulose chromatography of the
DNA polymerase
-primase-
C2 protein
complex resolves the
C2 protein
from a complex of
DNA polymerase alpha
-primase.
...
PMID:A multiprotein form of DNA polymerase alpha from HeLa cells. Resolution of its associated catalytic activities. 370 Apr 10
