Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.7 (
DNA polymerase
)
17,007
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The induction of
DNA polymerase
of herpes simplex virus type 2 in HeLa cells was inhibited by 2-mercapto-1-(
beta-4
-pyridethyl)benzimidazole added at an early time after infection. However, the inhibitory effect was easily released by removal of the inhibitor. Since the drug disorganizes nucleolar structure and prevents its function in HeLa cells in a reversible manner, it is possible that nucleoli are involved in the induction of
DNA polymerase
of this virus.
...
PMID:Reversible inhibition of the induction of DNA polymerase of herpes simplex virus type 2 in HeLa cells. 21 36
DNA replicase
activity of synchronized cultures of HeLa cells was assayed by a permeable cell technique during normal S-phase and under conditions of restricted RNA, protein, or DNA synthesis. Inhibition studies with puromycin, cycloheximide, actinomycin D, and 2-mercapto-1-(
beta-4
-pyridethyl)benzimidazole revealed that the establishment as well as the maintenance of
DNA replicase
activity in S-phase cells was dependent on the continued synthesis of both RNA and protein. Measurements during limitation of DNA replication by hydroxyurea, cytosine arabinoside, or restricted availability of thymidine indicate that a low level of DNA synthesis is required to activate or assemble some subunits of
DNA replicase
. Evidence for the existence of short-lived RNA and protein factors essential for
DNA replicase
activity is discussed.
...
PMID:A requirement for RNA, protein and DNA synthesis in the establishment of DNA replicase activity in synchronized HeLa cells. 111 85
