Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.7 (
DNA polymerase
)
17,007
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A simple, efficient procedure for cross-linking two complementary oligonucleotides, which does not require chemical modification of either oligonucleotide, is described. One of the oligonucleotides is first converted to the 5'-phosphorothioate derivative with polynucleotide kinase. It is then incubated with its complement in the presence of 1 microM trans-platinum(II)diammine dichloride. After overnight incubation, 40-50% cross-linking is observed. DNA synthesis by the
Klenow fragment
of Escherichia coli
DNA polymerase I
is blocked at the cross-linked site, resulting in the formation of truncated products.
Potassium platinous chloride
(K2PtCl4) and cis-platinum(II)diammine dichloride form cross-links less efficiently than the trans isomer.
...
PMID:A simple procedure for cross-linking complementary oligonucleotides. 218 Apr 30
