Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.7.7 (DNA polymerase)
 17,007 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Forty-three kb of DNA, located at the left end (45 to 88 kb) of the 330-kb Chlorella virus PBCV-1 genome, was sequenced and analyzed. Eighty-six open reading frames (ORFs) 65 codons or longer were identified; 47 were classified as major ORFs. These 47 major ORFs are densely packed on both strands of PBCV-1 DNA. Seventeen of these major ORFs resemble genes in the sequence databases, including three putative gene products involved in manipulating sugars (glucosamine synthetase, GDP-D-mannose dehydratase, and N-acetylglucosaminyltransferase), two transcription factors, beta-1,3-glucanase, aspartate transcarbamylase, ubiquitin carboxy terminal hydrolase, RNA guanyl transferase, an exonuclease, and a helicase. This is the first time some of these putative PBCV-1 genes have been found in a virus genome. One of the transcription factor-like genes contains a type IB self-splicing intron. Since a spliceosomal processed intron was reported previously in the PBCV-1 DNA polymerase gene, PBCV-1 is the first virus known to contain two different types of introns.
 ...
PMID:Analysis of 43 kb of the Chlorella virus PBCV-1 330-kb genome: map positions 45 to 88. 767 24

A design for recognition of beta-1,3-glucanase gene (Glu) specific sequence based on probe extension was described. The detecting probe DNA and the anchoring prober were hybridized with the same target DNA firstly, then the probes were extended by DNA polymerase reaction. After that the double strand DNA was denatured, and the extended detecting probe was immobilized on a glassy carbon electrode via nanoparticle gold (AuNP). In electrochemical detection (cyclic voltammetry, CV and differential pulse voltammetry, DPV), an increased peak current (i(p)) of the indicator (methylene blue, MB) was obtained compared with the probe without extension. Three differently long DNAs of Glu specific sequence were employed as the target: oligonucleotide acid, molecular cloning vector DNA and total genome DNA of transgenic capsicum. The estimated DPV detection limits for three targets of oligonucleotide, the molecular cloning vector DNA and genome DNA were 2.6x10(-13), 6.0x10(-13) and 8.0x10(-13)moll(-1) respectively.
 ...
PMID:Electrochemical detection of beta-1,3-glucanase gene from transgenic capsicums using asymmetric PCR generated by a detecting probe and an anchoring probe. 1979 43