Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.7.7 (
DNA polymerase
)
17,007
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The present work provides experimental evidence of two ways of fermentative degradation of DNA replication forks in vivo. The first way proceeds under the influence of
exonuclease V
, which degradates DNA replication forks, uncovered by singlestrand binding (ssb) proteins. DNA replication forks protected against nucleases by ssb protein are degrated by a second way: by tandem action of
DNA polymerase II
with ssb protein complexes and
exonuclease V
. The default of normal ssb protein in cells of Escherichia coli is responsible for the first way of DNA replication forks degradation. The second way is occurs at excess of normal ssb protein.
...
PMID:[Enzymatic mechanisms of degradation of DNA replication forks in vitro]. 639 99
Cell survival, the rates of DNA and protein synthesis, stabilization and repair of single-stranded DNA breaks were measured for Escherichia coli K-12 pol+, polA1, polB1, polC(ts) cells during thymine deprivation. The results indicate that single-stranded breaks in DNA induced by thymine deprivation play no major role in thymineless death of thy- polB- cells, which is similar to thy recB mutant lacking
exonuclease V
. Probably, the dependence of
exonuclease V
activity on the presence of
DNA polymerase II
(the polB gene product) exists which determines molecular mechanisms of thymineless death of thy- polB- cells.
...
PMID:[Effect of polB mutation on the nature of the thymineless death of thy- cells of Escherichia coli K-12]. 675 28
Homologous recombination is a fundamental biological process. Biochemical understanding of this process is most advanced for Escherichia coli. At least 25 gene products are involved in promoting genetic exchange. At present, this includes the RecA, RecBCD (
exonuclease V
), RecE (exonuclease VIII), RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, and SSB proteins, as well as
DNA polymerase I
, DNA gyrase, DNA topoisomerase I, DNA ligase, and DNA helicases. The activities displayed by these enzymes include homologous DNA pairing and strand exchange, helicase, branch migration, Holliday junction binding and cleavage, nuclease, ATPase, topoisomerase, DNA binding, ATP binding, polymerase, and ligase, and, collectively, they define biochemical events that are essential for efficient recombination. In addition to these needed proteins, a cis-acting recombination hot spot known as Chi (chi: 5'-GCTGGTGG-3') plays a crucial regulatory function. The biochemical steps that comprise homologous recombination can be formally divided into four parts: (i) processing of DNA molecules into suitable recombination substrates, (ii) homologous pairing of the DNA partners and the exchange of DNA strands, (iii) extension of the nascent DNA heteroduplex; and (iv) resolution of the resulting crossover structure. This review focuses on the biochemical mechanisms underlying these steps, with particular emphases on the activities of the proteins involved and on the integration of these activities into likely biochemical pathways for recombination.
...
PMID:Biochemistry of homologous recombination in Escherichia coli. 796 21
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